γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium usawoti Cas:63699-78-5 Impushana ephuzi ephuzi ekhanyayo
| Inombolo Yekhathalogi | XD90183 |
| Igama Lomkhiqizo | γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium usawoti |
| I-CAS | 63699-78-5 |
| I-Molecular Formula | I-C12H12N3O7-.H4N+ |
| Isisindo samangqamuzana | 328.27 |
| Imininingwane Yesitoreji | I-ambient |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana encane ephuzi ephuzi |
| U-Asay | 99% |
| Iphoyinti lokuncibilika | 187ºC ± 2.0ºC |
| Iphuzu elibilayo | 718.3°C ku-760 mmHg |
| Iphoyinti le-Flash | 368.5 °C |
| Ukuncibilika | I-H2O: 100 mg/mL, icacile, iphuzi-luhlaza |
I-kinetics ye-serum ye-gamma-glutamyltransferase (EC 2.3.2.2) yaphenywa, ngokusetshenziswa kwe-glycylglycine njenge-gamma-glutamyl yokwamukela i-substrate kanye ne-gamma-glutamyl-4-nitroanilide nokuphuma kwayo ku-carboxy, i-gamma-glutamyl-3-carboxy-4 -nitroanilide, njengama-substrates abanikeli.Ukwenzeka ngesikhathi esisodwa kwakho kokubili i-gamma-glutamyltransfer ne-autotransfer kwasungulwa ngokwehla kwe-chromatography yephepha.Iziqephu eziphindaphindekayo ezinesilinganiso esiphindwe kabili ziqinisekisa ukuthi indlela ye-enzyme ayinalutho (ping-pong bi-bi).Ukuvinjelwa yinoma yimuphi umnikezeli akutholakalanga, futhi ukuvinjelwa yi-glycylglycine kwaqashelwa kuphela ekugxilweni okungaphezu kwalokho okuthakaselwayo emtholampilo.Ama-kinetic constants atholwe ngokuhlaziywa kokuhlehla okungewona komugqa wedatha yesivinini sokuqala asetshenziselwa ukunquma ukugxilwa kwe-reagent substrate yokuhlolwa kwale enzyme.Ukuhlolwa okusetshenziselwa u-4 mmol we-gamma-glutamyl-3-carboxy-4-nitroanilide kanye no-100 mmol we-glycylglycine ilitha kuveze imisebenzi efanayo naleyo ngokuhlola kusetshenziswa u-4 mmol we-gamma-glutamyl-4-nitroanilide kanye no-40 mmol glycylglycine ilitha.Lokhu kugxila komnikeli we-carboxy kanye ne-glycylglycine nakho "kuyabiza kakhulu" futhi azikho izinkinga zenqubo lapho kusetshenziswa.
