I-bacterium entomopathogenic, i-Xenorhabdus nematophila, yenza ukuzivikela kwezinambuzane eziqondiwe ngokuvimbela umsebenzi we-phospholipase A(2) (PLA(2)).Muva nje, isakhi sofuzo se-PLA(2) esihlobene nokuzivikela sitholwe kubhungane likafulawa obomvu, i-Tribolium castaneum.Lolu cwaningo luhlanganise lesi sakhi sofuzo se-PLA(2) ku-vector ye-bacterial expression ukukhiqiza i-enzayimu ehlangene.I-recombinant T. castaneum PLA(2) (TcPLA(2)) ibonise umsebenzi wayo we-enzyme enokugxila kwe-substrate, i-pH, nezinga lokushisa elizungezile.Izici zayo ze-biochemical zihambisana nohlobo oluyimfihlo lwe-PLA(2) (sPLA(2)) ngoba umsebenzi wayo wawuvinjwe i-dithiothreitol (i-ejenti enciphisa i-disulfide bond) ne-bromophenacyl bromide (i-sPLA(2) inhibitor ethile) kodwa hhayi i-methylarachidonyl. i-fluorophosphonate (uhlobo oluthile lwe-cytosolic lwe-PLA(2)).Umhluzi we-X. nematophila culture wawuqukethe i-PLA(2) inhibitory factor(ama-s), eyayigcwele kakhulu kwabezindaba etholwe esigabeni sokukhula kwebhaktheriya esimile.I-PLA(2) inhibitory factor(ama) ibikwazi ukumelana nokushisa futhi ikhishwe kuzo zombili izingxenyana ezinamanzi neziphilayo.Umthelela wengxenyana ye-PLA(2)-inhibitory on the immunosuppression of T. castaneum iqhathaniswe ngokulinganayo naleyo ebangelwe ukuvinjelwa kwesisho sofuzo se-TcPLA(2) ngokuphazanyiswa kwe-RNA.
