2′-Deoxythymidine Cas:50-89-5
| Inombolo Yekhathalogi | XD90582 |
| Igama Lomkhiqizo | 2'-Deoxythymidine |
| I-CAS | 50-89-5 |
| I-Molecular Formula | I-C10H14N2O5 |
| Isisindo samangqamuzana | 242.23 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29349990 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana emhlophe ukuya kokumhlophe |
| Isivivinyo | 99% |
| Ukulahlekelwa Ekumisweni | <0.5% |
| Izinsalela ekushiseni | <0.3% |
| Ukujikeleza okuqondile okubonakalayo | +17.5 - +19.5 |
Ngaphambilini sihlangabezane nezinqubo zokulawula lapho i-dihydrotestosterone (DHT) isebenzise umphumela wayo ovimbelayo ekucindezelweni kofuzo kwe-parathyroid hormone-related protein (PTHrP) ngokusebenzisa i-estrogen receptor (ER)α, kodwa hhayi i-androgen receptor (AR), kumdlavuza webele we-MCF-7 amaseli. .Lapha, siphenye ukuthi ukusebenzisana okunjalo kwe-aberrant ligand-nuclear receptor (NR) kukhona yini kumaseli e-LNCaP omdlavuza wendlala yesinye.Okokuqala, siqinisekise ukuthi amaseli e-LNCaP aveze inani elikhulu le-AR emazingeni angenalutho we-ERα/β noma i-progesterone receptor.Kokubili ukucindezelwa kwe-PTHrP kanye nokusebenza kwezakhi zofuzo ze-prostate-specific antigen kwabonwa ngemva kokuphathwa okuzimele kwe-17β-estradiol (E2), DHT, noma i-R5020.Ngokuvumelana nombono wokuthi i-LNCaP AR ilahlekelwe ukucaciswa kwayo kwe-ligand ngenxa yokuguquka kwesimo (Thr-Ala877), ukuhlolwa kwe-siRNA okuqondiswe ku-NR efanele kwembule ukuthi i-AR yaphatha indima yomlamuleli wokulawulwa okwabiwe okuncike ku-hormone, okwakuhlotshaniswa ngokungaguquki. nge-nuclear translocatio n yale AR eguquguqukayo.Ukuhlaziywa kwe-Microarray yokulawulwa kofuzo nge-DHT, E2, noma i-R5020 kudalule ukuthi ngaphezu kwengxenye yezakhi zofuzo ezansi nomfula we-AR (Thr-Ala877) zigqigqe kumaseli e-LNCaP.Okuthakaselekayo, saqaphela ukuthi i-AR (uhlobo lwasendle [wt]) kanye ne-AR (Thr-Ala877) zazibophezele ngokulinganayo ekusebenzisaneni kwe-E2-AR.Ukuhlolwa kwe-Fluorescence microscopy kubonise ukuthi kokubili i-EGFP-AR (wt) ne-EGFP-AR (Thr-Ala877) zenziwe zasendaweni ngokukhethekile ngaphakathi kwe-nucleus ngemva kokwelashwa kwe-E2 noma i-DHT.Ngaphezu kwalokho, izivivinyo zezintatheli ziveze ukuthi amanye amangqamuzana omdlavuza akhombisa ukubonakaliswa kwe-E2-AR (wt) okufana nalokhu kumaseli e-LNCaP.Lapha sibeka ubukhona bokusebenzelana okubambene phakathi kwe-wt AR ne-E2 kumaseli athile omdlavuza azwela i-hormone.
