![3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98% Isithombe Esifakiwe](http://cdn.globalso.com/xdbiochems/75621-03-3.jpg)
3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98%
| Inombolo Yekhathalogi | XD90075 |
| Igama Lomkhiqizo | 3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate |
| I-CAS | 75621-03-3 |
| I-Molecular Formula | I-C32H58N2O7S |
| Isisindo samangqamuzana | 614.88 |
| Imininingwane Yesitoreji | I-ambient |
| Kuvunyelanisiwe Ikhodi Yentela | 29242970 |
Ukucaciswa Komkhiqizo
| IR | Iqhathaniswa nereferensi |
| Ukulahlekelwa Ekumisweni | <2% |
| I-TLC | Indawo eyodwa |
| I-nitrogen | 4.46 - 4.65 |
| Ukubukeka | Impushana emhlophe |
| Ukunyibilika (10 % ku-H2O) | Icacile futhi ayinambala |
| I-Conductivity (0.1M ku-H2O) | <10us/cm |
| I-Assay HPLC | >98% |
| I-UV (5% ku-H2O) A290nm | <0.5 |
| I-UV (5% ku-H2O) A400nm | <0.02 |
| I-Solvent Content (nge-GC) i-Dimethylformamide | <2.0% |
3--[3-(cholamidopropyl) dimethylamino] propanesulfonic acid ukusetshenziswa kukasawoti wangaphakathi kanye nendlela yokuhlanganisa
Umsebenzi Webhayoloji: I-CHAPS, ephuma ku-Cholicacid, isihlanzi se-zwitterionic esinyibilikisa amaprotheni olwelwesi.I-CHAPS isetshenziselwa ukuzinzisa ama-protein-DNA complexes ahlukahlukene futhi ingagcina umsebenzi wamakhemikhali we-biochemical wamaprotheni ekuxazululeni.
Isetshenziswa: I-CHAPS isihlanzi se-zwitterionic esingashisi i-membrane biochemistry.Ingasetshenziselwa ukuncibilikisa amaprotheni e-membrane futhi iphazamise ukusebenzisana kwamaprotheni namaprotheni.I-CHAPS inesisindo esiphansi semolekyuli ye-micellar (6,150) kanye nokugxila okuphezulu okubucayi kwe-micelle (6-10 mM) ngakho-ke ingasuswa kusampula nge-dialysis.Ibuye ilungele ukuxazulula amaprotheni ekugxilweni kwe-isoelectric kanye ne-2D electrophoresis.I-CHAPS ivamise ukusetshenziswa ekugxiliseni kogesi kwendabuko (okungenayo i-urea) futhi kutholakale ukuthi inikeza ukulungiswa okuhle kakhulu kwamalungiselelo athile angaphansi kwamaselula kanye namaprotheni ezitshalo.Ngokuvamile ukugxila okuphakathi kuka-2-4% (w/v) kusetshenziselwa amajeli agxilisa ingqondo kagesi.
Isebenzisa: Isihlanzi se-zwitterionic esingavali i-denaturing se-membrane biochemistry, esingasetshenziswa ukunyibilikisa amaprotheni olwelwesi lwama-membrane kanye nokuphazamisa ukusebenzisana kwamaprotheni-namaprotheni;futhi ifanele ukuxazulula amaprotheni ekugxilweni kwe-isoelectric kanye ne-electrophoresis enezinhlangothi ezimbili;evame ukusetshenziswa ekugxilweni kwe-Non-denaturing (ayikho i-urea) ye-isoelectric;Ukukhishwa kwe-RNA ne-DNA, akukho msebenzi we-RNase ne-DNase
Isetshenziswa: I-amphoteric surfactant, i-CHAPS isihlanzi se-zwitterionic esinga-denaturing, i-protein lysate yokuncibilikisa amaprotheni olwelwesi lwama-membrane kanye nokusebenzisana okuqamba amanga phakathi kwamaprotheni namaphrotheni.
