4-Amino-3-hydrazino-1,2,4-triazol-5-thiol Cas:28836-03-5 99% Okuqinile okuphuzi kuya kokuluhlaza
| Inombolo Yekhathalogi | XD90146 |
| Igama Lomkhiqizo | I-4-Amino-3-hydrazino-1,2,4-triazol-5-thiol |
| I-CAS | 28836-03-5 |
| I-Molecular Formula | C16H13NO3S·NH3 |
| Isisindo samangqamuzana | 316.37 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 2923900090 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Okuqinile okuphuzi kuya kokuluhlaza |
| U-Asay | ≥ 99% |
| Iphoyinti lokuncibilika | 237°C(dec.)(lit.) |
| Ukuncibilika | I-NaOH: i-soluble1 N |
| Amanzi ancibilikayo | Incibilika emanzini, i-1N NaOH, ne-methanol. |
1.Sifunde imiphumela evimbelayo ye-isorhamnetin ku-mushroom tyrosinase nge-inhibition kinetics kanye ne-computational simulation.I-Isorhamnetin ivimbele ukubuyisela emuva i-tyrosinase ngendlela yohlobo oluxubile kokuthi Ki=0.235±0.013 mM.Izilinganiso ze-fluorescence ezibopha ngaphakathi kanye ne-1-anilinonaphthalene-8-sulfonate(ANS) zibonise ukuthi i-isorhamnetin ayizange yenze izinguquko ezibalulekile esakhiweni semfundo ephakeme se-tyrosinase.Ukuze uthole ukuqonda ngenqubo yokungasebenzi, ama-kinetics abalwa kusetshenziswa izilinganiso zesikhawu sesikhathi kanye nokusabela kwe-substrate okuqhubekayo.Imiphumela ibonise ukuthi ukungasebenzi okubangelwa i-isorhamnetin kwaba ukusabela kwe-oda lokuqala ngezinqubo ze-biphasic.Ukuze sithole ukuqonda okwengeziwe, silingise ukudotshwa kwedokodo phakathi kwe-tyrosinase ne-isorhamnetin.Ukulingisa kube yimpumelelo (amandla okubopha ku-Dock6.3: -32.58 kcal/mol, ku-AutoDock4.2: -5.66 kcal/mol, naku-Fred2.2: -48.86 kcal/mol), okuphakamisa ukuthi i-isorhamnetin isebenzisana nezinsalela ezimbalwa, ezifana njenge-HIS244 kanye ne-MET280.Leli su lokusebenzelana kwe-predic ting tyrosinase lihlanganiswe ne-kinetics esekelwe kwinhlanganisela ye-flavanone lingase libe usizo ekuhloleni ama-tyrosinase inhibitors emvelo angaba khona.
2.Indlela evula i-Acid ye-conalbumin (CA), i-monomeric glycoprotein evela kumhlophe kweqanda lesikhukhukazi, iphenywe kusetshenziswa i-spectroscopy ye-CD ekude naseduze ne-UV, i-intrinsic fluorescence emission, i-extrinsic fluorescence probe 1-anilino-8-napthalene sulfonate (Anilino-8-napthalene sulfonate) kanye nokusabalalisa ukukhanya okunamandla (DLS).Sibona izinguquko ezincike ku-pH kusakhiwo sesibili nesiphezulu se-CA.Inesakhiwo sesibili esinjenge-α-helical ku-pH 4.0 kodwa ukwakheka kokulahlekelwa ku-pH 3.0.I-CA ibikhona ngokukhethekile njengendawo yeglobula yangaphambi kokuncibilika kanye nesimo seglobule esincibilikisiwe esixazululweni ku-pH 4.0 kanye ne-pH 3.0, ngokulandelana.Umthelela we-pH ekuhambisaneni nasekushiseni kwe-CA ukhomba ekumelaneni nokushisa kwayo nge-pH emaphakathi.Imiphumela ye-DLS ibonisa ukuthi isimo se-MG sasikhona njengefomu elihlangene ezixazululweni ezinamanzi ane-hydrodynamic radii engu-4.7 nm.Ukucinywa kwe-tryptophan fluorescence nge-acrylamide kuphinde kwaqinisekisa ukuqoqwa kwesimo esimaphakathi, esingasombulukile ngokwengxenye, phakathi kwezifunda zomdabu nezingaguquki.
![N-[[bis[4-(dimethylamino)phenyl]amino]carbonyl] glycine sodium usawoti Okumhlophe kuya kokumpunga okuluhlaza ngokumpunga okuyikristalu](http://cdn.globalso.com/xdbiochems/115871-19-7.jpg)
