Imikhiqizo

Ukugxila kwe-submillimolar ye-cytotoxic bile acid (BAs) kudala ukufa kwamaseli nge-apoptosis.Ngakolunye uhlangothi, ama-BA amaningana e-cytoprotective aboniswa ukuvimbela i-apoptosis ebangeni lokuhlushwa elifanayo.Noma kunjalo, izindlela ama-BA aqala ngazo le miphumela yokubonisa okuphambene zihlala zingacacile.Lolu cwaningo beluhloselwe ukunquma ukuthi ama-BA e-cytotoxic kanye ne-cytoprotective, ekugxilweni okusebenzayo kwe-physiologically, ayakwazi ukulungisa izakhiwo ze-biophysical zama-membrane e-lipid, okungenzeka ahumushele izinguquko embundwini we-apoptotic wamaseli.Ukuboshelwa kwama-BA kulwelwesi kwahlolwa ngokuhlukahluka kwemingcele ye-fluorescence yama-BA afanelekile akhishwe.Lokhu okuphuma kokunye kuhlukaniswe ngokusondelana okuphezulu koketshezi oluguquguqukayo kunezizinda ezi-odwe eziwuketshezi ezicebile nge-cholesterol.Ama-BA angenamalebula aphinde aboniswa anendawo ekha phezulu lapho ehlangana nolwelwesi lwe-lipid.Ukwengeza, ukusebenzisana kwe-cytotoxic BAs nolwelwesi kuphumele ekwandeni kolwelwesi, njengoba kuhlanganiswe kudatha ye-FRET.Ngaphezu kwalokho, kwaboniswa ukuthi ama-BA e-cytotoxic akwazi ukuphazamisa kakhulu ukuhleleka kwe-membrane nge-cholesterol ekugxilweni okusebenzayo kwe-BA, umphumela ongahlotshaniswa nokususwa kwe-cholesterol.Ngakolunye uhlangothi, i-cytoprotective bile acids ayizange ibe nomthelela ku-membrane izakhiwo.Kwaphethwa ngokuthi, uma kubhekwa imiphumela ekuqineni kolwelwesi, umsebenzi we-apoptotic wama-cytotoxic BAs ungase uhlotshaniswe nezinguquko ekuhleleni ulwelwesi lwe-plasma (isb. ukuguquguquka kwezizinda ze-lipid) noma nokwanda kokuhlangana kolwelwesi lwe-mitochondrial lwamaprotheni e-apoptotic.