4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4 Off-White Powder 98%
| Inombolo Yekhathalogi | XD90011 |
| Igama Lomkhiqizo | 4-Nitrophenyl-alpha-D-mannopyranoside |
| I-CAS | 10357-27-4 |
| I-Molecular Formula | I-C12H15NO8 |
| Isisindo samangqamuzana | 30301.25 |
| Imininingwane Yesitoreji | -2ku-8°C |
| Ikhodi Yentela Evumelanisiwe | 29400000 |
Ukucaciswa Komkhiqizo
| Amanzi | <5% Karl Fische |
| Ukuncibilika | I-1% ku-DMF icacile futhi ayinambala |
| Ubumsulwa | I-4-Nitrophenol <200ppm yamahhala |
| I-HPLC | >98% |
| Ukubukeka | Impushana emhlophe |
Imininingwane yemishini emndenini oncike ku-Ca2+ wama-alpha-mannosidase ku-symbiont yamathumbu omuntu.
Amagciwane angamakholoni, aboniswa yi-Bacteroides thetaiotaomicron, adlala indima ebalulekile ekugcineni impilo yomuntu ngokuhlanganisa imindeni emikhulu yama-glycoside hydrolases (GHs) ukuze asebenzise ama-polysaccharides okudla futhi abambe ama-glycans njengezakhi.Ukwandiswa komndeni we-GH okunjalo kuboniswa umndeni we-23 we-GH92 glycosidase ofakwe nge-B. thetaiotaomicron genome.Lapha sibonisa ukuthi lawa angama-alpha-mannosidase asebenza ngendlela eyodwa yokususa ukuze asebenzise i-N-glycans engusokhaya.Isakhiwo esinezinhlangothi ezintathu se-GH92 mannosidase sichaza umndeni wamaphrotheni anezizinda ezimbili lapho isikhungo se-catalytic sitholakala ku-interface yesizinda, sihlinzeka ngosizo lwe-asidi (glutamate) nesisekelo (aspartate) ku-hydrolysis ku-Ca(2+) - indlela encike.Izakhiwo ezinezinhlangothi ezintathu ze-GH92 eziyinkimbinkimbi ezinama-inhibitors zinikeza ukuqonda mayelana nokucaciswa, indlela kanye nohambo oluhambisanayo lwe-catalysis.I-Ca(2+) idlala indima ebalulekile ekusizeni ukuhlanekezela i-mannoside kude ne-ground-state yayo (4)C(1) ukuhambisana kwesitulo esibheke kusimo soguquko.（I-Bibliography: Nat.I-Chem.I-Biol.6, 125-32, (2010)
I-Frontal affinity chromatography ye-ovalbumin glycoasparagines kukholomu ye-concanavalin A-sepharose.Ucwaningo lwenani lokucaciswa okubophezelayo kwe-lectin.
Ukusebenzisana kwe-Sepharose 4B-immobilized concanavalin A (ConA) nama-glycoasparagines ayi-10 atholakala ku-ovalbumin kwaphenywa ngobuningi nge-frontal affinity chromatography.Kule ndlela, isisombululo se-carbohydrate sisetshenziswa ngokuqhubekayo kukholomu ye-ConA-Sepharose futhi ukuhlehliswa kwe-lution front kukalwa njengepharamitha yamandla okuxhumana.I-dissociation constant (Kd) ye-saccharide ngayinye ene-ConA inganqunywa.Ukuhlaziywa kokubophezela kwe-p-nitrophenyl-alpha,D-mannoside kubonise ukuthi izici ezibophayo ze-ConA azishintshi ngokuyisisekelo ngemva kokufakwa kwe-Sepharose 4B.I-ovalbumin glycoasparagines ngayinye yayibhalwe ukuthi i-tritium ngendlela yokunciphisa i-methylation yokuhlaziywa.Ukuqhathaniswa kwamanani e-Kd atholiwe kubonise ukuthi ukubophezela kwe-ConA kuyahluka kakhulu ngokuhluka okuncane kakhulu kwesakhiwo seketango le-glycosyl.Imiphumela iphakamisa ukuthi i-ConA ibona ukwakheka kweketango elithile le-glycosyl, i-Man alpha 1-6(Man alpha 1-3)Man, lapho okungenani iqembu elilodwa le-hydroxyl endaweni ye-C-3 ye-mannose exhunywe ku-C-6 kufanele likhululeke.
