Imikhiqizo

I-tRNA iyisigaba esilungiswe kakhulu sezinhlobo ze-RNA, futhi ukuguqulwa kutholakala kuma-tRNA avela kuzo zonke izinto eziphilayo ezihloliwe.Naphezu kwezakhiwo zazo zamakhemikhali ezihluke kakhulu kanye nokuba khona kwazo kuma-tRNA ahlukene, okwenzeka ezindaweni ezihlukene ku-tRNA, izindlela ze-biosynthetic zoningi lokuguqulwa kwe-tRNA zifaka phakathi isinyathelo(izinyathelo) se-methylation.Okutholwe kwakamuva kwembule inkimbinkimbi engakaze ibonwe kumaphethini okuguqulwa kwe-tRNA, ukulawula nokusebenza kwayo, okuphakamisa ukuthi i-nucleoside ngayinye eshintshiwe ku-tRNA ingase ibe nomsebenzi wayo othize.Nokho, ezitshalweni, ulwazi lwethu ngendima yokuguqulwa kwe-tRNA ngayinye kanye nendlela elawulwa ngayo lulinganiselwe kakhulu.Esikrinini sofuzo esiklanyelwe ukuhlonza izici ezilawula ukumelana nezifo nokwenza kusebenze izivikelo ku-Arabidopsis, sihlonze i-SUPPRESSOR OF CSB3 9 (SCS9).Imiphumela yethu yembula i-SCS9 efaka ikhodi ye-tRNA methyltransferase elamula i-2'-O-ribose methylation yezinhlobo ezikhethiwe ze-tRNA ku-antico don loop.Lokhu kuguqulwa kwe-tRNA okune-SCS9-mediated kuyathuthuka ngesikhathi sokutheleleka nge-pathogen yebhaktheriya i-Pseudomonas syringae DC3000, kanye nokuntuleka kokuguqulwa okunjalo kwe-tRNA, njengoba kuphawulwe kuma-mutants we-scs9, kubeka engcupheni ukuvikeleka kwezitshalo ngokumelene ne-pathogen efanayo ngaphandle kokuthinta uphawu lwe-salicylic acid (SA) indlela elawula ukusabela kwamasosha omzimba ezitshalo.Imiphumela yethu isekela imodeli enikeza ukubaluleka ekulawuleni izinguquko ezithile ze-tRNA ukuze kukhuliswe impendulo ephumelelayo yokuzivikela komzimba ku-Arabidopsis, futhi ngenxa yalokho inwebe iqoqo lezingxenye zamangqamuzana ezibalulekile ekuphenduleni okuphumelelayo kokumelana nezifo.