Imikhiqizo

I-Neuroblastoma, umdlavuza wesimiso sezinzwa esinozwela, iyithumba eliqinile elivela ngaphandle kwe-cranial ezinganeni.Ukukhulisa i-MYCN nokwenyuka kokusayina kwe-BDNF/TrkB kuyizici zamathumba ayingozi kakhulu;kodwa, ngu-˜25% kuphela wamathumba amabi abonisa lezi zici.Ngakho-ke, ukukhonjwa kwama-biomarker engeziwe kanye nezinhloso zokwelapha kubalulekile.Njengoba i-aminoacylase 1 (ACY1), i-amino acid deacetylase, iyisicindezeli sesimila esibulalayo kungqamuzana elincane lamaphaphu kanye ne-renal cell carcinomas, siphenye ukuthi ingabe yona noma amanye amalungu omndeni i-aspartoacylase (ASPA, aminoacylase 2) noma i-aminoacylase 3 (ACY3) ingasebenza. umsebenzi ofanayo ku-neuroblastoma.Isisho se-aminoacylase sihlolwe ku-TrkB-positive, MYCN-amplified (SMS-KCNR ne-SK-N-BE) kanye ne-TrkB-negative, non-MYCN-amplified (SK-N-AS, SK-N-SH, SH-SY5Y kanye SH-EP) imigqa yeseli ye-neuroblastoma.I-aminoacylase ngayinye ibonise ukwenziwa kwasendaweni okuhlukile (okungukuthi, i-cytosolic ACY1, i-ASPA ehambisana nolwelwesi kanye ne-nuclear ACY3).Lapho amaseli e-SK-N-SH ephathwa nge-neural differentiation agents (isb, i-retinoic acid ne-cAMP) emithonjeni yezindaba equkethe i-serum engu-10%, i-ACY1 iyona kuphela i-aminoacylase ukuvezwa kwayo kwakulawulwa.I-ASPA ivezwe ngokuyinhloko ngamaseli e-SH-EP we-glial sublineage.I-ACY3 yavezwa kakhulu emigqeni ye-TrkB-positive, MYCN-amplified.Womathathu ama-aminoacylases abonakaliswe ku-adrenal gland yomuntu evamile, indawo evamile yomsuka we-neuroblastoma, kodwa i-ACY1 ne-ACY3 kuphela ebonise ukubonakaliswa okubonakalayo kusimila esiyinhloko se-neuroblastoma.Ukumbiwa kwedatha ye-Bioinformatics kokusinda kwe-Kaplan-Meier kwembula ukuthi ukusho okuphezulu kwe-ACY3 kuhlotshaniswa nokubikezela okubi, kanti ukubonakaliswa okuphansi kwe-ACY1 noma i-ASPA kuhlotshaniswa nokubikezela okubi.Le datha iphakamisa ukuthi inkulumo ye-aminoacylase i-dysregulated ku-neuroblastoma