Imikhiqizo

1.Ukuphenya ukuthi ingabe i-adenosine diphosphate (ADP)-derived adenosine ingase ivimbele ukuhlangana kweplatelet, ikakhulukazi uma kukhona ophikisana naye we-P2Y₁₂, lapho imiphumela ye-ADP ku-P2Y₁₂ receptor izovinjelwa.Ukuhlangana kwePlatelet kukalwe ngokuphendula i-activatorn receptor i-peptide ngokubala kweplatelet ku-plasma ecebile ye-platelet (PRP) kanye negazi eliphelele phambi kwe-ADP kanye ne-P2Y₁₂ antagonists cangrelor, prasugrel metabolite esebenzayo, ne-ticagrelor.Ebukhoneni bomphikisi we-P2Y₁₂, i-preincubation ye-PRP ene-ADP ehlanganisiwe evinjelwe;lo mphumela waqedwa yi-adenosine deaminase.Akukho ukuvinjelwa kokuhlangana okwenzeka egazini lonke ngaphandle kwalapho i-dipyridamole yengezwa ukuze kuvinjwe ukutholwa kwe-adenosine kuma-erythrocyte.Imiphumela ye-ADP ku-PRP kanye negazi eliphelele laphindwa kabusha kusetshenziswa i-adenosine futhi yayihlobene ngokuqondile nezinguquko ku-cAMP (ihlolwe yi-vasodilator-stimulated phosphoprotein phosphorylation).Yonke imiphumela ibifana ngokunganaki kwe-antagonist ye-P2Y₁₂ esetshenzisiwe.I-ADP ivimbela ukuhlangana kweplatelet phambi komphikisi we-P2Y₁₂ ngokuguqulelwa ku-adenosine.Ukuvinjelwa kwenzeka ku-PRP kodwa hhayi egazini lonke ngaphandle uma ukumuncwa kwe-adenosine kuvinjiwe.Abekho abaphikisi be-P2Y₁₂ abahloliwe abaphindaphinda imiphumela ye-dipyridamole ekuhlolweni okwenziwe.

2.I-ADP ithathwa njenge-agonist ebuthakathaka ye-platelet ngenxa yezimpendulo ezilinganiselwe ezihlanganisayo ezingenisa ku-vitro ekugxilweni kokuphila kwe-Ca(2+) engaphandle kwe-extracellular Ca(2+) [(Ca(2+) )(o) ].Ukwehlisa [Ca(2+) ](o) kuthuthukisa ngendlela exakayo ukuhlanganisa okuvusa i-ADP, umthelela obalulwe ekukhiqizeni okuthuthukisiwe kwe-thromboxane A(2).Lolu cwaningo luhlole indima ye-ectonucleotidases ku- [Ca (2+) ](o) -ukuxhomekeka kokusebenza kweplatelet.Ukunciphisa [i-Ca(2+) ](o) kusuka kumazinga e-millimolar kuya ku-micromolar aguqule i-ADP (10 μmol/l) -okuvusa ukuhlanganiswa kweplatelet ukusuka kwesikhashana kuya ekuphenduleni okuqhubekayo kukho kokubili i-plasma ecebile ngamaplatelet kanye nokumiswa okugeziwe.Ukuvimbela ukukhiqizwa kwe-thromboxane A(2) nge-aspirin akuzange kube nomthelela kulokhu [Ca(2+) ](o) -dependence.Ukuvinjelwa kokucekelwa phansi kwe-ADP kuqede umehluko phakathi kokuphansi nokokusebenza komzimba [Ca(2+) ](o) okuholela ekuhlanganisweni okuqinile nokuqhubekayo kuzo zombili izimo.Izilinganiso ze-ADP engaphandle kwe-extracellular zembule ukuwohloka okunciphile kukho kokubili i-plasma ne-apyrase-containin g saline ku-micromolar uma kuqhathaniswa ne-millimolar [Ca(2+) ](o) .Njengoba kubikiwe ngaphambilini, isizukulwane se-thromboxane A(2) siye sathuthukiswa ngokuphansi [Ca(2+) ](o) , nokho lokhu bekuzimele kumsebenzi we-ectonucleotidase(.) I-P2Y receptor antagonists kanye ne-MRS2179 ibonise isidingo sama-receptors e-P2Y(12) ngokuhlanganisa okuqhubekayo kwe-ADP-evukile, nendima encane ye-P2Y(1) .Sengiphetha, i-Ca(2+) -umsebenzi we-ectonucleotidase othembele kuyisici esiyinhloko esinquma izinga lokuhlanganiswa kweplatelet ku-ADP futhi kufanele ilawulwe ezifundweni zokuvula i-P2Y receptor.