I-monoclonal antibody (mAb 8281) eqondene netheminali i-β-galactose (βGal) ye-glycosphingolipids (GSL) nama-glycoprotein akhiqizwa kusukela kumagundane agonywe nge-lipid ekhishwe kumaseli amasha acute lymphocytic leukemia (ALL).I-Immuno-thin layer chromatography (ITLC) kanye nokuhlolwa kokuncintisana okunamazinga e-GSL ahlanzekile angathathi hlangothi, ushukela wamahhala, nama-synthetic neoglycoprotein abonise i-mAb 8281 ukuthi isebenze ngokuqinile nge-LacCer, GalCer ne-Gal-β-O-(CH3)2S(CH3)2- I-CONH-(Gal-β-O-CETE) exhunywe ku-albumin ye-bovine serum (BSA).Ushukela ongaphambi kwesikhathi nawo ube neqhaza ekubopheni.I-antibody yayingasebenzi ngama-carbohydrate anezakhiwo ze-αGal zokugcina kanye nezingxenye zesiphetho ezingahlobene.I-immunoperoxidase staining and flow cytometry ene-mAb 8281 ibonise amabala amahle ezicutshini eziningi, okuhlanganisa imisipha ebushelelezi, i-gastrointestinal mucosa, amaseli e-lymph node B nama-monocyte.Ukuhlaziywa kwe-ITLC kokubunjwa kwe-GSL kwe-B cell neoplasms entsha esebenzisa i-mAb 8281 kuqinisekisile ukuba khona kwe-lactosylceramide ne-galactosylceramide kuma-neoplasms ezigaba ezihlukene zokuhlukanisa.Ngenxa yokucaciswa kwayo kwezinsalela ze-βGal carbohydrate, i-mAb 8281 ingase ibe usizo ekuhlaziyeni kwesakhiwo nokusebenza kwe-GSL.
