Imikhiqizo

I-Bromelain (BR) iyi-cysteine ​​protease enemiphumela evimbelayo ekukhiqizweni kwamathumbu nokuvuvukala.Kodwa-ke, imiphumela yawo ekuhambeni kwamathumbu ayikahlolisiswa.Ngakho-ke, siphenye umthelela wale nhlanganisela ethathwe esitshalweni ekuvumelwaneni kwamathumbu kanye nokuhamba ngamagundane.I-Contractility in vitro yahlolwa ngokugqugquzela ileum ehlukanisiwe yegundane, endaweni yokugeza yesitho, nge-acetylcholine, i-barium chloride, noma isivuseleli senkundla kagesi.I-Motility ku-vivo ikalwe ngokuhlola ukusatshalaliswa komaka olawulwa ngomlomo we-fluorescent eduze kwamathumbu amancane.I-Transit nayo yahlolwa ezifundeni ze-pathophysiologic ezibangelwa amafutha e-croton e-pro-inflammatory noma i-agent ye-diabetogenic streptozotocin.I-Bromelain ivimbele ukufinyela okubangelwa ama-compounds ahlukene e-spasmogenic ku-ileum yegundane enamandla afanayo.Umphumela we-antispasmodic wehlisiwe noma uphikiswe yi-proteolytic enzyme inhibitor, i-gabexate (15 × 10(-6) mol L(-1)), i-protease-activated r eceptor-2 (PAR-2) antagonist, N (1) -3 -methylbutyryl-N(4) -6-aminohexanoyl-piperazine (10(-4) mol L(-1)), i-phospholipase C (PLC) inhibitor, neomycin (3 × 10(-3) mol L(-1) ) , kanye ne-phosphodiesterase 4 (PDE4) inhibitor, i-rolipram (10(-6) mol L(-1)).Ku-vivo, i-BR ivimbele ngokukhethekile ukuhambahamba ezimeni ze-pathophysiologic ngendlela ezwela i-PAR-2-antagonist.Idatha yethu iphakamisa ukuthi i-BR ivimbela ukuhamba kwamathumbu - ngokukhethekile ezimeni ze-pathophysiologic - ngomshini okungenzeka ukuthi uhilela ulwelwesi i-PAR-2 ​​ne-PLC kanye ne-PDE4 njenge-intracellular izimpawu.I-Bromelain ingaba isakhi esiholayo sokuthuthukiswa kwemithi emisha, ekwazi ukujwayela ukuhamba kwamathumbu ekuvuvukala nesifo sikashukela.