Imikhiqizo

Inqubo yokungcolisa ichazwa ukuze isetshenziswe nezingxenye zethishu ezishumekiwe ze-glycol methacrylate ezingangcolisi okushumekiwe kwepulasitiki noma zisuse izigaba kumaslayidi engilazi.Idayi eyisisekelo i-celestine eluhlaza okwesibhakabhaka B. Ilungiselelwe ngokuphatha i-1 g yedayi nge-0.5 ml egxilile ye-sulfuric acid.Ibe isichithwa nesixazululo esilandelayo.Engeza u-14 ml we-glycerine ku-100 ml 2.5% i-ferric ammonium sulfate bese ufudumeza ikhambi libe ku-50 C. Ekugcineni lungisa i-pH ibe ngu-0.8 kuya ku-0.9 Isixazululo se-acid staining siqukethe u-0.075% we-ponceau de xylidine no-0.025% we-acid fuchsintic ku-asidi engu-10%.Amaslayidi aqukethe izingxenye zepulasitiki ezomile acwiliswa kusixazululo esiluhlaza okwesibhakabhaka se-celestine imizuzu emihlanu kanye nesisombululo se-ponceau-fuchsin imizuzu eyishumi ngokugeza kwamanzi okungenele.Ngemva kokugeza okokugcina, izingxenye zomiswa emoyeni futhi zimbozwe.Le nqubo yokungcolisa imibala izicubu icishe ifane nezinqubo ze-hematoxylin ne-eosin.