Imikhiqizo

Ama-brucellae ama-etiological agents of brucellosis, i-zoonosis esatshalaliswa emhlabeni wonke.Lawa mabhaktheriya angama-facultative intracellular parasites futhi ngaleyo ndlela ayakwazi ukulungisa i-metabolism yawo endaweni engaphezulu kanye ne-intracellular ahlangabezane nayo phakathi nomjikelezo othathelwanayo.Kodwa-ke, lesi sici se-Brucella biology asiqondakali kahle, futhi imisoco etholakala ku-intracellular niche ayaziwa.Lapha, siphenye izindlela ezimaphakathi ze-C metabolism esetshenziswa i-Brucella abortus ngokususa i-puative fructose-1,6-bisphosphatase (fbp ne-glpX), i-phosphoenolpyruvate carboxykinase (pckA), i-pyruvate phosphate dikinase (ppdK), ne-enzyme ye-malic (mae) ufuzo.Ku-gluconeogenic kodwa hhayi emithonjeni yezindaba ecebile, ukukhula kwe-ΔppdK kanye ne-Δmae mutants kukhubazeke kakhulu futhi ukukhula kwe-Δfbp-ΔglpX eguquguqukayo kabili kwehlisiwe.Kuma-macrophages, kuphela i-ΔppdK ne-Δmae mutants ebonise ukuphindaphinda okuncishisiwe, futhi izifundo ne-ΔppdK mutant ziqinisekisile ukuthi zifinyelele niche yokuphindaphinda.Ngokufanayo, kuphela i-ΔppdK ne-Δmae mutants eyancishiswa ngamagundane, eyokuqala yasulwa ngeviki le-10 futhi yokugcina yaqhubeka isikhathi eside kunamaviki e-12.Siphinde saphenya umjikelezo we-glyoxylate.Nakuba umsebenzi womgqugquzeli we-aceA (isocitrate lyase) uthuthukisiwe endaweni enothile, ukuphazamiseka kwe-aceA akuzange kube nomthelela ku-vitro noma ekuphindaphindeni kwama-macrophage noma imibende yegundane.Imiphumela iphakamisa ukuthi i-B. abortus ikhula ngaphakathi kwe-intracellular isebenzisa ukunikezwa okulinganiselwe kwe-6-C (kanye no-5-C) ushukela onxeshezelwa yi-glutamate futhi mhlawumbe namanye ama-amino acid angena kumjikelezo we-Krebs ngaphandle kwendima ebalulekile ye-glyoxylate shunt.