I-Chymotrypsinogen A CAS:9035-75-0 I-crystaline powder emhlophe
| Inombolo Yekhathalogi | XD90376 |
| Igama Lomkhiqizo | I-Cymotrypsinogen A |
| I-CAS | 9035-75-0 |
| I-Molecular Formula | I-C47H58FeN4O4S2 |
| Isisindo samangqamuzana | 862.96 |
| Imininingwane Yesitoreji | -20°C |
Ukucaciswa Komkhiqizo
| Isivivinyo | 99% |
| Ukubukeka | I-crystalline powder emhlophe |
Ukwengezwa kwamaphrotheni ezindaweni zokusebenzelana zoketshezi oluqinile kuvame ukutholakala ngokunamandla ukuze kuthuthukiswe ukwakheka kwamaqoqo ancibilikayo kanye nezinhlayiya ezinkulu, ezibonakalayo, nezibonakalayo, kodwa izigaba ezibalulekile kule nqubo ngokuvamile kuba nzima ukuziphenya ngokuqondile.Ukuhlanganiswa okuxhunywe nge-adsorption ku-water-silicon oxide (SiOx) interface, efana nendawo yengilazi enamanzi, kuphawulwe njengomsebenzi we-pH namandla e-ionic we-alpha-chymotrypsinogen (aCgn) kanye ne-monoclonal antibody (IgG1).Iseli eligelezayo livumele ukubonakaliswa kwe-neutron kwezendlalelo zamaprotheni ezikhangiselwe ukuhlanza izindawo ze-SiOx, kanye nangemva kwezinyathelo "zokugeza" ezilandelanayo.Ama-aggregate atholwe njengesixazululo ngemva “kokugeza” ngobumnene indawo engaphezulu abonakale ngokusakazwa kwe-neutron, i-microscopy, kanye ne-fluorescence spectroscopy.Ama-molecule e-IgG1 aqondiswe ikakhulukazi "esicaba" endaweni ye-SiOx, ungqimba lwamaprotheni oluyinhloko luhlukaniswe ngokwezinga elincane, kanti isendlalelo esisabalalisiwe sasulwa kalula.Ama-aCgn ama-molecule ayemelana nokuncibilika lapho ebonakala sengathi angambuleka kusixhumi esibonakalayo, kodwa asuswa kalula.Ezimeni lapho ukubopha okuqinile kwenzeke khona, iphrotheni eyenza i-desorb yayiyingxube ye-monomer kanye namanani amancane we-HMW aggregates (ye-aCgn) noma izinhlayiya ezingabonakali (ye-IgG1).Izinguquko ekukhangisweni kanye/noma ekuvulekeni nge-pH zibonise ukuthi ukusebenzisana kwe-electrostatic bekubalulekile kuzo zonke izimo.
