Imikhiqizo

I-Spiral tube assembly counter-current chromatography isetshenziswe ngempumelelo ekuhlukaniseni i-dl-tryptophan kusetshenziswa i-albumin ye-bovine serum njengesikhethi se-chiral.Uhlelo oluthuthukisiwe lwe-biphasic aqueous-aqueous solvent 12.0% (w/w) i-polyethyleneglycol 8000-9.0% (w/w) i-dibasic potassium phosphate-0.1% ammonia-78.9% amanzi asetshenziswe njengesistimu yokuncibilikisa ye-counter-current chromatography, lapho i-albumin ye-bovine serum yasatshalaliswa kakhulu esigabeni esiphansi sesistimu ye-aqueous yezigaba ezimbili.Uhlelo lwe-solvent enamanzi ane-aqueous-aqueous lunikeze i-enantioselectivity ephezulu kakhulu ye-d- ne-l-tryptophan kokuthi α=2.605 kanye nesilinganiso sokusabalalisa DD=1.200 kanye ne-DL=0.461.Kutholwe ukulungiswa okuphezulu okuphezulu ukuze kuhlukaniswe i-2.0mg ye-dl-tryptophan nge-spiral tube assembly counter-current chromatography ngaphansi kwezinga lokushisa legumbi.Kutholwe ukuthi i-0.1% ye-ammonia engezwe ohlelweni lwe-aqueous-aqueous solvent ithuthukise kakhulu ukuhlukaniswa.Ukuphakama okungavamile okubanzi kakhulu kwe-l-tryptophan kwabonwa ngesikhathi se-en antioseparations.Ukuze kunikezwe incazelo, izilinganiso zokudlulisa ngobuningi zika-d- kanye ne-l-enantiomers ngokusebenzisa isixhumi esibonakalayo phakathi kwezigaba ezimbili ziye zalinganiswa.Kutholakale ukuthi i-l-tryptophan ibonise izinga eliphansi lokudluliswa kwesisindo kune-d-tryptophan.Izingxoxo ezengeziwe zahlongozwa ngezizathu ezingaba khona zomehluko wesilinganiso sokudlulisa ngobuningi phakathi kwama-enantiomers.