I-Dithizone CAS: 60-10-6 80% Impushana eluhlaza okwesibhakabhaka
| Inombolo Yekhathalogi | XD90464 |
| Igama Lomkhiqizo | I-Dithizone |
| I-CAS | 60-10-6 |
| I-Molecular Formula | I-C13H12N4S |
| Isisindo samangqamuzana | 256.33 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29309098 |
Ukucaciswa Komkhiqizo
| Ukulahlekelwa Ekumisweni | ≤5% |
| Ukubukeka | Impushana eluhlaza okwesibhakabhaka |
| Isivivinyo | 80% |
| Izinsalela ekushiseni | ≤0.2% |
I-Mesenchymal stem cells (MSCs) kubikwe ukuthi iwumthombo okhangayo wokukhiqiza amaseli e-β adluliselwa komunye umuntu.Umugqa weseli weseli ye-embryonic mesenchymal progenitor C3H10T1/2 ubonwe njengemodeli yama-MSC, ngenxa yamandla awo okuhlukanisa imigqa eminingi.Inhloso yalolu cwaningo bekuwukuhlola ukuthi ingabe amaseli e-C3H/10T1/2 anamandla okuhlukanisa abe amaseli akhiqiza i-insulin (IPCs).Lapha, siphenye futhi saqhathanisa ukuhlukaniswa kwe-in vitro kwamagundane ama-MSC namaseli e-C3H10T1/2 kwaba ama-IPC.Ngemuva kokuthi amaseli ethole ukuhlukaniswa kwe-IPC, ukuvezwa komaka bokuhlukanisa kutholwe nge-immunocytochemistry, reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time RT-PCR (qRT-PCR) kanye ne-Western blotting.Ukukhiqizwa kwe-insulin kwahlolwa kusetshenziswa i-enzyme-linked immunosorbent assay (ELISA).Ngaphezu kwalokho, lawa maseli ahlukanisiwe atshalwa kabusha kumagundane anesifo sikashukela enziwe yi-streptozotocin futhi imisebenzi yawo yebhayoloji yahlolwa ku-vivo.Lolu cwaningo lubika indlela yezigaba ezimbili yokukhiqiza ama-IPC kusuka kumaseli e-C3H10T1/2.Ngaphansi kwezimo ezithile zokungeniswa kwezinsuku ezingu-7-8, amaseli e-C3H10T1/2 akha imizimba ye-spheroid enezinhlangothi ezintathu (ama-SBs) kanye ne-insulin eyimfihlo, kuyilapho ukukhiqizwa kwama-IPC atholakala kumagundane ama-MSC adinga isikhathi eside (ngaphezu kwamaviki angu-2).Ngaphezu kwalokho, lawa ma-IPC athathwe kumaseli e-C3H10T1/2 ajovwa kumagundane anesifo sikashukela futhi athuthukisa i-basal glucose, isisindo somzimba futhi abonise ukuhlolwa okujwayelekile kokubekezelela ushukela.Ucwaningo lwamanje lunikeze imodeli ye-in vitro elula nethembekile ukuze kuqhutshekwe nokuphenya indlela engaphansi kwe-IPC yokuhlukaniswa kwama-MSC kanye nokwelashwa okushintshwa kwamaseli kwesifo sikashukela.
