Imikhiqizo

I-N(ε)-trifluoroacetyl-L-lysine iwukuphuma okuthile kwe-amino acid lysine, lapho iqembu le-epsilon (ε) amino livikelwa yiqembu le-trifluoroacetyl (TFA).Le nhlanganisela ithola ukusetshenziswa ezindaweni ezihlukahlukene, ikakhulukazi ku-peptide synthesis kanye nocwaningo lwamakhemikhali ezinto eziphilayo.Okunye ukusetshenziswa okukhulu kwe-N(ε)-trifluoroacetyl-L-lysine is in solid-phase peptide synthesis (SPPS).Ama-peptide adlala indima ebalulekile ezinqubweni eziningi zezinto eziphilayo, futhi ukwakheka kwawo kudinga ukuhlanganiswa okulandelanayo kwama-amino acid.Iqembu elivikelayo le-TFA eqenjini le-epsilon amino le-lysine livimbela ukusabela okungafuneki ngesikhathi sokwakheka kwebhondi ye-peptide kuyilapho livumela amanye ama-amino acid ukuthi ashade ezindaweni ezifiselekayo.Uma ukuhlanganiswa kwe-peptide sekuqediwe, iqembu elivikelayo le-TFA lingasuswa ngokukhetha, futhi izinsalela ze-N(ε)-trifluoroacetyl-L-lysine zinganwetshwa ngokuqhubekayo ukuze kuhlanganiswe ukulandelana kwe-peptide ethile.N(ε)-trifluoroacetyl-L-lysine nayo isetshenziswe ocwaningweni lwe-biochemical ukutadisha ukwakheka kwamaprotheni, ukusebenza, kanye nokusebenzisana.Ingasebenza njengethuluzi lokuphenya indima yezinsalela ze-lysine ekusebenzisaneni kwamaprotheni-amaprotheni, ukusebenzisana kwamaprotheni-DNA, kanye nokuguqulwa kwangemuva kokuhumusha.Ngokwethula iqembu le-TFA, izakhiwo zamakhemikhali e-lysine zingashintshwa, okuvumela uphenyo lwezinqubo ezithile ze-lysine-mediated ezinhlelweni zebhayoloji.Ngaphezu kwalokho, i-N(ε)-trifluoroacetyl-L-lysine ingathola futhi ukusetshenziswa ekuthuthukisweni kokuhlosiwe. izinhlelo zokulethwa kwezidakamizwa.Ngokuhlanganisa ama-ejenti athile okwelapha ezinsalela ze-lysine ngeqembu le-TFA, kungenzeka ukwandisa ukuzinza nokukhetha komuthi, okuholela ekusebenzeni okuthuthukisiwe kanye nemiphumela emibi encishisiwe.Lelisu liye lahlolisiswa ekulethweni kwezidakamizwa ezilwa nomdlavuza, ama-antibiotics, nezinye izindlela zokwelapha.Ngaphezu kokusetshenziswa kwayo okuqondile, i-N(ε)-trifluoroacetyl-L-lysine ingasetshenziswa njengesakhiwo sokuhlanganiswa kwezinye izinhlanganisela ezisebenzayo. .Ukwakheka kwayo kwamakhemikhali okuyingqayizivele nokusebenza kabusha kuyenza ibe yinto yokuqala ekhangayo noma emaphakathi ekuhlanganiseni kwama-molecule ezinto eziphilayo eziyinkimbinkimbi, okuhlanganisa imithi, amakhemikhali ezolimo, nezinto zokwakha. Noma kunjalo, kubalulekile ukuphatha ngokucophelela i-N(ε)-trifluoroacetyl-L-lysine, ngokulandela izinyathelo zokuphepha ezifanele.Kunconywa ukusebenza elabhorethri engenisa umoya futhi usebenzise izinto zokuzivikela ezifanele, njengamagilavu ​​nezibuko zokuphepha. Kafushane, i-N(ε)-trifluoroacetyl-L-lysine iwukuphuma okubalulekile kwe-lysine okuthola ukusetshenziswa okuhlukahlukene ku-peptide. i-synthesis, ucwaningo lwe-biochemical, ukulethwa kwezidakamizwa okuhlosiwe, kanye ne-organic synthesis.Ikhono layo lokuvikela iqembu le-epsilon amino le-lysine livumela ukwakheka kwe-peptide okunembile, kuyilapho izakhiwo zayo zamakhemikhali eziguquliwe zivumela ukucwaninga kwezinqubo ze-lysine-mediated.Uma iphathwe kahle, i-N(ε)-trifluoroacetyl-L-lysine ibonakala iyithuluzi eliwusizo ezinhlelweni ezihlukahlukene zesayensi nezimboni.