Imikhiqizo

I-Islet amyloid polypeptide (IAPP; eyaziwa nangokuthi i-amylin) inesibopho sokwakheka kwe-islet amyloid ohlotsheni lwesifo sikashukela sohlobo 2, futhi ubuthi obubangelwa yi-IAPP kukholakala ukuthi bunomthelela ekulahlekeni kwesisindo se-β-cell ehambisana nezigaba zakamuva zohlobo 2 lwesifo sikashukela.Ukwakheka kwe-Islet amyloid kungase futhi kudlale indima ekuhlulekeni kokuxhunyelelwa ngemva kokufakelwa kabusha.I-IAPP ikhiqizwa njenge-prohormone, i-pro-islet amyloid polypeptide (proIAPP), futhi icutshungulwe kuzinhlamvana eziyimfihlo zamaseli e-pancreatic β.Izinhlobo ezicutshungulwe kancane ze-proIAPP zitholakala kumadiphozithi e-amyloid;okuphawuleka kakhulu i-48-residue intermediate, i-proIAPP(1-48), ehlanganisa isandiso se-N-terminal pro-extension, kodwa esicutshungulwe kahle ku-C-terminus.Ukucubungula okungaphelele kungase kubambe iqhaza ekwakhekeni kwe-islet amyloid ngokukhuthaza ukusebenzisana ne-sulfated proteoglycans ye-extracellular matrix, yona, ekhuthaza ukwakheka kwe-amyloid.Sibonisa ukuthi i-acid fuchsin (3-(1-(4-amino-3-methyl-5-sulphonatophenyl)-1-(4-amino-3-sulphonatophenyl)methylene)cyclohexa-1,4-dienesulphonic acid), i-asidi elula i-sulfonated triphenyl methyl derivative, iyisivimbeli esinamandla sokwakheka kwe-amyloid yi-proIAPP(1-48) emaphakathi.Okuphuma kokuphuma ku-triphenyl methane okuyinkimbinkimbi kakhulu okuluhlaza okushesha kwe-FCF {ethyl-[4-[[4-ethyl-[(3-sulfophenyl)methyl]amino]phenyl]-(4-hydroxy-2-sulfophenyl)methylidene]-1-cyclohexa -2,5-dienylidene]-[(3-sulfophenyl)methyl]azanium} futhi ivimbela ukwakheka kwe-amyloid yi-IAPP kanye nokucubungula kwe-proIAPP okumaphakathi.Zombili izinhlanganisela zivimbela ukwakheka kwe-amyloid ngezingxube ze-proIAPP emaphakathi kanye nemodeli ye-glycosaminoglycan heparan sulfate.I-Acid fuchsin iphinda ivimbele ukwakheka kwe-amyloid ye-glycosaminoglycan nge-IAPP ekhulile.Amandla okuvimbela ukwakheka kwe-amyloid akukona nje ngenxa yokuthi izinhlanganisela zi-sulfonated, njengoba i-sulfonated inhibitor ye-amyloid-β, tramiprosate, ayiyona inhibitor yokwakheka kwe-amyloid nge-proIAPP(1-48).