Imikhiqizo

Sakhe inoveli encane ephazamisayo yohlelo lwe-RNA (siRNA) sisebenzisa i-ternary complex ene-polyethyleneimine (PEI) ne-γ-polyglutamic acid (γ-PGA), ebonise umphumela wokuthulisa futhi ayikho i-cytotoxicity.Izinkimbinkimbi kanambambili ze-siRNA ezine-PEI zazicishe zibe ngu-73-102 nm ngosayizi wezinhlayiyana kanye no-45-52 mV ku-ζ-amandla.Umphumela wokuthulisa we-siRNA/PEI complexes ukhuphuke ngokunyuka kwe-PEI, futhi izakhiwo ze-siRNA/PEI ezinesilinganiso sokushaja esikhulu kune-16 zibonise ukwehla okuphawulekayo kwe-luciferase kumugqa weseli we-mouse colon carcinoma ehlala eveza i-luciferase (amaseli e-Colon26/Luc).Kodwa-ke, i-cytotoxicity eqinile kanye ne-blood agglutination yabonwa ku-siRNA/Lipofectamine complex kanye ne-siRNA/PEI16.Ukushajwa kabusha kwezakhiwo ze-cationic ngenhlanganisela ye-anionic kubikwe ukuthi kuyindlela ethembisayo yokunqoba lobu buthi.Ngakho-ke silungise ama-ternary complexes e-siRNA nge-PEI (isilinganiso seshajiji engu-16) ngokufaka i-γ-PGA ukuze kwehliswe i-cytotoxicity futhi ilethe i-siRNA.Njengoba bekulindelekile, i-cytotoxicity ye-ternary complexes yehla ngokunyuka kokuqukethwe kwe-γ-PGA, okwehlise u-ζ-amandla wezakhiwo.Umphumela oqinile wokuthulisa ofana nenkimbinkimbi ye-siRNA/Lipofectamine watholwa ezindaweni eziphakeme ezihlanganisa i-γ-PGA ene-anionic surface charge.Ukufakwa okuphezulu kwama-ternary complexes kumaseli e-Colon26/Luc kwaqinisekiswa nge-fluorescence microcopy.Ngemva kokuthola ukudilizwa kofuzo oludluliselwe ngendlela exakile, ikhono lenkimbinkimbi lokulamula ukudilizwa kofuzo lokugcinwa kwendlu, i-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), lahlolwa kumaseli e-B16-F10.I-ternary complex (siRNA/PEI16/γ-PGA12 complex) ibonise umthelela wokuwisa we-GAPDH.Ngakho, sakha uhlelo oluwusizo lokulethwa kwe-siRNA.