I-Guanidine Hydrochloride Cas: 50-01-1 99% Impuphu emhlophe
| Inombolo Yekhathalogi | I-XD90198 |
| Igama Lomkhiqizo | I-Guanidine Hydrochloride |
| I-CAS | 50-01-1 |
| I-Molecular Formula | I-CH6N3Cl |
| Isisindo samangqamuzana | 95.5388 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29252900 |
Ukucaciswa Komkhiqizo
| I-Melting Point | 185.3 - 187.5 Deg C |
| Amanzi | <0.3% |
| Ukuncibilika | Sula isixazululo esingenambala ku-6M Emanzini |
| Isivivinyo | >99% |
| I-UV Absorbance | 0.10 ku-260nm |
| Ukubukeka | Impushana emhlophe |
| I-spectrum ye-IR | Kuyavumelana |
Esinye sezici ezivimbela ukusetshenziswa komtholampilo komuthi ovuselelayo ezifweni ze-cartilage eziwohlokayo umthombo ofanelekile wamaseli.Ama-Chondrocyte, okuwukuphela kohlobo lweseli loqwanga, olukhule ku-vitro ngaphansi kwezimo zesiko ukuze kwandiswe izinombolo zamaseli alahlekelwa yi-phenotype yawo kanye nekhono lokukhiqiza izicubu ze-hyaline cartilaginous.Kulolu cwaningo sinquma ukuthi uhlelo lwesiko lwe-serum- kanye ne-growth-free-factor-free-factor-free-three-dimensional (3D) lubuyisela ikhono lama-chondrocyte adlulile ukuze enze izicubu ze-cartilage in vitro, inqubo ehilela i-sox9.I-Bovine articular chondrocyte yadluliswa kabili ukuze vumela ukunwetshwa kwenombolo yeseli (P2) futhi ikhule ngokuminyana okuphezulu kuma-membrane e-3D collagen-type-II-coated emithonjeni yokuqukethwe kweglucose ephezulu engezwe nge-insulin ne-dexamethasone (SF3D).Amaseli abonakaliswe ngemuva kokunwetshwa kwe-monolayer nokulandela isiko le-3D ngokugeleza kwe-cytometry, i-gene expression, ne-histology.Izinguquko zakuqala emigwaqweni yokudlulisela izimpawu ngesikhathi se-ion yokuhlukanisa kabusha ziye zabonakala.Amaseli e-P2 abonise iphrofayili ye-antigen efana ne-progenitor engu-99% CD44 (+) kanye ne-40% ye-CD105 (+) kanye nephrofayili yesisho sofuzo esiphakamisa amaseli e-interzone.I-P2 ku-SF3D iveze izakhi zofuzo ze-chondrogenic kanye ne-matrix engaphandle kwamaseli anqwabelene.Yehlisa i-insulin receptor (IR) nge-HNMPA-(AM3) noma indlela ye-PI-3/AKT kinase (eyenziwe yasebenza ngokwelashwa kwe-insulin) nge-Wortmannin inhibited collagen synthesis.I-HNMPA-(AM3) inciphise ukuvezwa kwezakhi zofuzo ze-Col2, i-Col11, ne-IR kanye ne-Sox6 ne- -9.Ukuhlaziywa kwe-Co-immunoprecipitation kanye ne-chromatin immunoprecipitation yamaseli alashwe nge-HNMPA-(AM3) kubonise ukubophezela kwama-coactivator i-Sox6 ne-Med12 nge-Sox9 kodwa kunciphise ukubopha kwe-Sox9-Col2a1.Sichaza indlela yesiko elinoveli elivumela ukwanda kwenani lama-chondrocyte futhi likhuthaze ukwakheka kwezicubu ze-hyaline-like cartilage ingxenye ngokubophezela kwe-Sox9-Col2a1 kwe-insulin.Ukufaneleka kwezicubu ezikhiqizwe ngale ndlela ukuze zisetshenziswe ekulungiseni amalunga kufanele kuhlolwe ku-vivo.
