Imikhiqizo

I-infrared spectroscopy isetshenziselwe ukwenza imephu ukusebenzisana kwe-substrate-protein: izinguquko ezihambisanayo ze-sarcoplasmic reticulum Ca(2+)-ATPase phezu kokubopha kwe-nucleotide kanye ne-ATPase phosphorylation kwaqashwa kusetshenziswa i-substrate ATP ne-ATP analogue (2'-deoxy-ATP, 3 '-deoxy-ATP, ne-inosine 5'-triphosphate), eziye zashintshwa emaqenjini athile asebenzayo e-substrate.Ukuguqulwa kwe-2'-OH, i-3'-OH, kanye neqembu le-amino le-adenine kunciphisa izinga loshintsho lokuvumelana olubophezelayo lwe-ATPase, ngemiphumela eqinile ikakhulukazi ebonwa kokubili kokugcina.Lokhu kubonisa ukuzwela kwesakhiwo se-nucleotide-ATPase eyinkimbinkimbi ekusebenzisaneni komuntu ngamunye phakathi kwe-nucleotide ne-ATPase.Wonke amaqembu afundwayo abalulekile ekubopheni nasekuxhumaneni kweqembu elinikeziwe le-ligand ne-ATPase kuncike ekusebenzelaneni kwamanye amaqembu e-ligand.I-phosphorylation ye-ATPase yaqashelwa ku-ITP kanye ne-2'-deoxy-ATP, kodwa hhayi i-3'-deoxy-ATP.Akukho ukuxhumana okuqondile phakathi kwesilinganiso soshintsho oluhambisanayo ekubopheni i-nucleotide kanye nezinga le-phosphorylation elibonisa ukuthi izinga eligcwele loshintsho lwe-ATP lwe-conformational aluphoqelekile ku-phosphorylation.Njengoba kuqashelwa inkimbinkimbi ye-nucleotide-ATPase, ukwakheka kwe-phosphorylated ATPase emaphakathi E1PCa(2) yokuqala nakho kuncike ku-nucleotide, okubonisa ukuthi izifunda ze-ATPase zinokufana okuncane kunalokho obekulindelwe ngaphambilini.