L-Asparagine anhydrous CAS:70-47-3 99% Impuphu emhlophe
| Inombolo Yekhathalogi | XD90314 |
| Igama Lomkhiqizo | I-D-Aspartic acid |
| I-CAS | 70-47-3 |
| I-Molecular Formula | I-C4H8N2O3 |
| Isisindo samangqamuzana | 132.12 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29241900 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana emhlophe |
| Isivivinyo | 99% |
| Ukuzungezisa okuqondile | +34,2 ukuze +36,5 |
| Izinsimbi ezinzima | <10ppm |
| AS | <1ppm |
| pH | 4.4 - 6.4 |
| I-SO4 | <0.020% |
| Fe | <10ppm |
| Ukulahlekelwa Ekumisweni | <0.5% |
| NH4 | <0.10% |
| Cl | <0.020% |
| Isimo Sesixazululo | >98% |
I-MSMEG_0307 ichazwe njengesilawuli sokubhaliwe somndeni wamaprotheni we-AraC futhi itholakala eduze kofuzo lwe-arylamine N-acetyltransferase (nat) ku-Mycobacterium smegmatis, kuqoqo lofuzo, elondolozwe ezinhlotsheni eziningi ze-mycobacterial zemvelo.Ukuze kucaciswe umsebenzi wephrotheni ye-AraC kusukela ku-nat operon ku-M. smegmatis, ama-palindromic DNA motifs amabili alondoloziwe ahlonzwe kusetshenziswa i-bioinformatics futhi ahlolelwa ukubopha amaprotheni kusetshenziswa ama-electrophoretic mobility shift assay anefomu le-recombinant ye-AraC protein.Sihlonze ukwakheka kwe-DNA:AraC protein complex ene-motif eyodwa kanye nokuba khona kwale motif ku-20 loci kuyo yonke igenome ye-M. smegmatis, isekela ukuba khona kwe-AraC controlled regulon.Ukuveza imiphumela ye-AraC ekulawuleni izakhi zofuzo ze-nat operon, kanye nokuthola ukuqonda okwengeziwe ngomsebenzi wayo, sikhiqize uhlobo oluguquguqukayo lwe-ΔaraC lapho isakhi sofuzo se-araC sathathelwa indawo umaka wokuma we-hygromycin.Izinga lokuvezwa kwezakhi zofuzo ze-nat ne-MSMEG_0308 lalilawulwa phansi kuhlobo lwe-ΔaraC uma liqhathaniswa nohlobo lwasendle olubonisa umthelela we-activator wephrotheni ye-AraC ekuvezeni kofuzo lwe-nat operon.
