Imikhiqizo

I-EAAT2 glutamate transporter, yenza >90% we-hippocampal glutamate ethathayo.Nakuba i-EAAT2 ivezwa kakhulu kuma-astrocyte, ~10% wama-molecule e-EAAT2 atholakala kumatheminali e-axon.Ngaphandle kwezinga eliphansi lenkulumo ye-EAAT2 kumatheminali e-glutamatergic, lapho izingcezu ze-hippocampal zifakwe ngokugxiliswa okuphansi kwe-d-aspartate (i-EAAT2 substrate), amatheminali e-axon aqongelela i-d-aspartate ngokushesha njenge-astroglia.Lokhu kusho ukungafani okungachazwanga phakathi kokusatshalaliswa kwephrotheni ye-EAAT2 kanye nomsebenzi wezokuthutha owenziwe nge-EAAT2.Inkolelo-mbono eyodwa iwukuthi (1) ukushintshaniswa kwe-heteroexchange ye-substrate yangaphakathi ne-substrate yangaphandle kushesha kakhulu kunokuthatha inetha futhi (2) amatheminali athanda ukushintshaniswa kwe-heteroexchange ngenxa yamazinga aphezulu e-glutamate yangaphakathi.Kodwa-ke, okwamanje akwaziwa ukuthi i-heteroexchange nokuthatha kunezilinganiso ezifanayo noma ezihlukile.Ukuze sibhekane nale nkinga, sisebenzise isistimu ehlanganiswe kabusha ukuze siqhathanise izilinganiso ezihambisanayo zezinqubo ezimbili kumagundane namagundane.Ukuthathwa kwensalela kuye kwazwela ezinguqukweni zekhono le-membrane futhi kwakhuthazwa ama-anion angaphandle avunyelekayo ngokuvumelana nokuba khona kokuqhutshwa kwe-anion okungahlanganisiwe.Ngokusebenzisa lokhu kwakamuva, sibonisa futhi ukuthi izinga le-heteroexchange lincike emandleni olwelwesi.Ukwengeza, idatha yethu iphakamisa nokuba khona kokuvuza kwe-sodium ku-EAAT2.Ngokuhlanganisa okutholakele okusha kumodeli yethu yangaphambilini yokuthatha i-glutamate nge-EAAT2, sibikezela ukuthi ukuzwela kwe-voltage yokushintshana kubangelwe ukubophezela kwe-Na(+) yesithathu okuncike ku-voltage.Ngaphezu kwalokho, kokubili ukuhlola kwethu nokulingiswa kuphakamisa ukuthi amanani ahlobene okuthathwa kwensalela kanye ne-heteroexchange ayaqhathaniswa ku-EAAT2.