I-L-lysine dihydrochloride Cas: 657-26-1 99% I-crystalline powder emhlophe
| Inombolo Yekhathalogi | XD90307 |
| Igama Lomkhiqizo | I-L-lysine dihydrochloride |
| I-CAS | 657-26-1 |
| I-Molecular Formula | C6H14N2O2.2[HCl] |
| Isisindo samangqamuzana | 219.10944 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29224100 |
Ukucaciswa Komkhiqizo
| I-Melting Point | 200 - 205 Deg C |
| Amanzi | <0.5% |
| Ukujikeleza kwe-Optical | +15,9 |
| Ukubukeka | I-crystalline powder emhlophe |
| I-Assay (Titration ex Chloride) | >99% |
Ukuqoqwa kwe-nuclear yohlobo oluguquguqukayo lwephrotheni yenuzi i-Lamin-A, ebizwa ngokuthi i-Progerin (PG) noma i-Lamin AΔ50, kwenzeka ku-Hutchinson-Gilford Progeria Syndrome (HGPS) noma i-Progeria, isifo sokuguga esisheshayo.Esinye sezibonakaliso eziyinhloko zalesi sifo ukulahlekelwa kwamafutha angaphansi kwesikhumba ngenxa ye-lipodystrophy ephawulekayo. Senze ngokuzinza ukuvezwa kwe-PG yomuntu kanye ne-GFP -Green Fluorescent Protein- njengokulawula kumaseli angu-3T3L1 kusetshenziswa uhlelo lwe-lentviral ukufunda umphumela wokuvezwa kwe-PG kumthamo wokuhlukanisa walo mugqa weseli, enye yamamodeli asetshenziswa kakhulu e-adipogenic.I-Quantitative proteomics (iTRAQ) yenziwa ukutadisha umphumela wokuqoqwa kwe-PG.Amaprotheni amaningana ashintshiwe aqinisekiswa yi-immunoblotting kanye ne-PCR yesikhathi sangempela.Umsebenzi we-Mitochondrial wahlaziywa ngokulinganisa a) umsebenzi wesisekelo se-mitochondrial, b) ukukhiqizwa kwe-anion ye-superoxide kanye c) nokusebenza kahle komuntu ngamunye kwenhlanganisela ehlukene yeketango lokuphefumula. ukwehla kwezinga lokusabalala kanye nokukhubazeka kumthamo we-adipogenic uma kuqhathaniswa nokulawula.Ukuhlaziywa kwe-quantitative proteomics kubonise amaprotheni ayi-181 ngokuphawulekayo (p<0.05) ahlelwe kuma-PG-expressing preadipocyte.Ukusebenza kwe-Mitochondrial konakala kumaseli aveza i-PG.Ngokuqondile, sithole ukwanda komsebenzi we-complex I kanye nokukhiqizwa ngokweqile kwe-anion ye-Superoxide.I-Incubation with Reactive Oxygen Species (ROS) ama-scavenger agents ishayela ekwehleni kwe-autophagic proteolysis njengoba kwembulwa yi-LC3-II/LC3-I ratio.PG expression in 3T3L1 cells ikhuthaza izinguquko Ezinqubweni Zebhayoloji ezimbalwa, okuhlanganisa ukwakheka kwe-cytoskeleton, i-lipid metabolism, i-calcium. ukulawulwa, ukuhumusha, ukugoqa amaprotheni kanye nokukhiqizwa kwamandla nge-mitochondria.Idatha yethu iqinisa umnikelo wokuqoqwa kwe-ROS ku-phenotype yokuguga ngaphambi kwesikhathi futhi isungula isixhumanisi phakathi kokungasebenzi kahle kwe-mitochondrial nokulahlekelwa kwe-proteostasis ku-HGPS.
