I-L-Proline Cas: 147-85-3 99% Impuphu emhlophe
| Inombolo Yekhathalogi | XD90293 |
| Igama Lomkhiqizo | I-L-Proline |
| I-CAS | 147-85-3 |
| I-Molecular Formula | I-C5H9NO2 |
| Isisindo samangqamuzana | 115.13046 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29339980 |
Ukucaciswa Komkhiqizo
| Isivivinyo | 99%imizuzu |
| Ukubukeka | Impushana emhlophe |
| Ukuzungezisa okuqondile | -84.5 kuya ku-86 |
| Izinsimbi ezinzima | <15ppm |
| AS | <1ppm |
| Ph | 5.9 - 6.9 |
| I-SO4 | <0.050% |
| Fe | <30ppm |
| Ukulahlekelwa Ekumisweni | <0.3% |
| Izinsalela ekushiseni | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| Isimo Sesixazululo | >98% |
Ukuqonda i-metabolism ye-microbial host kubalulekile ekuthuthukisweni nasekusetshenzisweni kahle kwezinqubo ze-biocatalytic ezisekelwe kumaseli wonke, njengoba ilawula ukusebenza kahle kokukhiqiza.Lokhu kuyiqiniso ikakhulukazi nge-redox biocatalysis lapho kusetshenziswa amaseli asebenzayo ngenxa ye-cofactor/cosubstrate regenerative umthamo we-endo native kumsingathi.I-Recombinant Escherichia coli yasetshenziselwa ukukhiqiza ngokweqile i-proline-4-hydroxylase (P4H), i-dioxygenase eyenza i-hydroxylation ye-L-proline yamahhala ibe yi-trans-4-hydroxy-L-proline ene-a-ketoglutarate (a-KG) njenge-cosubstrate.Kule biocatalyst yeseli lonke, i-carbon metabolism emaphakathi inikeza i-cosubstrate a-KG edingekayo, ehlanganisa ukusebenza kwe-P4H biocatalytic ngqo ku-carbon metabolism kanye nomsebenzi we-metabolic.Ngokusebenzisa kokubili amathuluzi ebhayoloji okuhlola nawokuhlanganisa, afana nobunjiniyela be-metabolic kanye (13) nokuhlaziywa kwe-C-metabolic flux ((13)C-MFA), siphenyile futhi sachaza ngokwesilinganiso impendulo yomzimba, ye-metabolic, kanye ne-bioenergetic ye-whole-cell biocatalyst. ekuguqulweni kwe-bioconversion okuhlosiwe futhi kuhlonzwe izingqinamba ezingaba khona ze-metabolic ukuze kuthuthukiswe ubunjiniyela bendlela enengqondo.Uhlobo lwe-E. coli oluntula ukuwohloka kwe-proline lwakhiwa ngokususa i-puA gene encoding proline dehydrogenase.I-whole-cell biotransformations enalolu hlobo lwe-mutant ayiholelanga nje kuphela ku-quantitative proline hydroxylation kodwa futhi ekuphindaphindeni kwesilinganiso sokwakheka kwe-trans-4-L-hydroxyproline (hyp), uma kuqhathaniswa nohlobo lwasendle.Ukuhlaziywa kwe-carbon flux ngokusebenzisa i-metabolism emaphakathi ye-mutant strain yembula ukuthi ukukhuphuka kwesidingo se-A-KG somsebenzi we-P4H akuzange kuthuthukise ukugeleza kokukhiqiza i-a-KG, okubonisa ukusebenza komjikelezo we-TCA olawulwa ngokuqinile ngaphansi kwezimo ezifundwayo.Ohlelweni lohlobo lwasendle, ukuhlanganiswa kwe-P4H kanye ne-catalysis kubangele ukuncipha kwesivuno se-biomass.Kuyathakazelisa ukuthi uhlobo lwe-ΔputA luphinde lwanxephezela ukulahlekelwa okuhlobene kwe-ATP ne-NADH ngokunciphisa izimfuno zamandla esondlo ngamanani aphansi ngokuqhathaniswa okuthatha i-glucose, esikhundleni sokukhulisa umsebenzi we-TCA.I- putA knockout in recombinant E. coli BL21(DE3)(pLysS) itholwe thembisa ukukhiqiza i-P4H catalysis hhayi kuphela ngokwesivuno se-biotransformation, kodwa futhi mayelana namazinga e-biotransformation kanye nokuthatha i-proline kanye nesivuno se-hyp emthonjeni wamandla.Imiphumela ikhombisa ukuthi, ekukhishweni kwe-putA, ukuhlangana komjikelezo we-TCA ukuze kusetshenziswe i-hydroxylation nge-cosubstrate a-KG kuba isici esibalulekile esicindezelayo kanye nokuhlosiwe ukuze kuthuthukiswe ukusebenza kahle kwe-biotransformations encike ku-KG.
