Imikhiqizo

Ubucayi bokumelana namagciwane kuhlanganiswe nokuntuleka kwama-antimicrobial amasha kukhulise intshisekelo ekuthuthukisweni kwe-antimicrobial peptides (AMPs) njengokwelashwa okunoveli.Kulolu cwaningo, sihlole imisebenzi ye-antimicrobial yama-peptide amabili amafushane okwenziwa, okungukuthi, i-RRIKA ne-RR.Lawa ma-peptide abonise umsebenzi onamandla we-antimicrobial ngokumelene ne-Staphylococcus aureus, futhi imiphumela yawo ye-antimicrobial yathuthukiswa kakhulu ngokungezwa kwama-amino acid amathathu ku-C terminus, okwathi ngenxa yalokho kwandisa i-amphipathicity, i-hydrophobicity, ne-net charge.Ngaphezu kwalokho, i-RRIKA ne-RR zibonise umphumela obalulekile futhi osheshayo wokubulala amagciwane ngokumelene ne-Staphylococcus isolate yomtholampilo nengazweli emuthini, okuhlanganisa ne-methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-intermediate S. aureus (VISA), S. aureus (VRSA) engazweli vancomycin , i-linezolid-resistant S. aureus, kanye ne-Staphylococcus epidermidis engazweli methicillin.Ngokuphambene nama-AMP amaningi emvelo, i-RRIKA ne-RR zigcine umsebenzi wendlalifa ebukhoneni bokugxila kokuphila kwe-NaCl ne-MgCl2.Kokubili i-RRIKA ne-RR kuthuthukise ukubulawa kwe-lysostaphin izikhathi ezingaphezu kwe-1,000 futhi kwaqeda ukuhlukaniswa kwe-MRSA ne-VRSA phakathi nemizuzu engama-20.Ngaphezu kwalokho, ama-peptide ethulwa abengcono ekwehliseni ama-biofilm anamathelayo e-S. aureus ne-S. epidermidis uma kuqhathaniswa nemiphumela ngama-antibiotic avamile.Ukuthola kwethu kubonisa ukuthi imiphumela ye-staphylocidal yama-peptide ethu yayibangelwa ukungena kwe-membrane yebhaktheriya, okuholela ekuvuzeni kokuqukethwe kwe-cytoplasmic nokufa kwamangqamuzana.Ngaphezu kwalokho, ama-peptide ayengenabo ubuthi kumaseli we-HeLa ku-4- kuya ku-8-fold ukugxila kwawo kwe-antimicrobial.Imisebenzi elwa namagciwane enamandla nengazweli usawoti yalawa ma-peptide iveza ikhandidethi elikhangayo lokwelapha ekwelapheni izifo eziningi ze-S. aureus ezimelana nezidakamizwa.