Usawoti wesodium we-MOPS Cas:71119-22-7 99.0% Impushana egelezayo yamahhala emhlophe
| Inombolo Yekhathalogi | XD90080 |
| Igama Lomkhiqizo | Usawoti we-sodium we-MOPS |
| I-CAS | 71119-22-7 |
| I-Molecular Formula | I-C7H14NNaO4S |
| Isisindo samangqamuzana | 231.245 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29349990 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Impushana egelezayo yamahhala emhlophe |
| Okuqukethwe kwamanzi KF | <1% |
| I-Assay (I-Titration, isisekelo esomile | >99.0% |
Ukuhlolwa kwe-chromatography ye-liquid entsha esebenza kahle kakhulu ye-glycosyltransferase esekelwe ekukhishweni kwe-anthranilic acid nokutholwa kwe-fluorescence.
Ukuhlola kwakhiwe kusetshenziswa ikhemikhali yokulebula eyingqayizivele ye-2-aminobenzoic acid (2AA; anthranilic acid, AA) ukuze kukale imisebenzi yakho kokubili i-β1-4 galactosyltransferase (GalT-1) kanye ne-α2-6 sialyltransferase (ST-6) ngoketshezi olusebenza kahle kakhulu. i-chromatography (HPLC) ngokutholwa kwe-fluorescence (Anumula KR. 2006. Intuthuko ezindleleni zokuphuma kwe-fluorescence zokuhlaziywa kwe-chromatographic ye-liquid ephezulu yokusebenza kwama-carbohydrate e-glycoprotein. I-Anal Biochem. 350:1-23).I-N-Acetylglucosamine (GlcNAc) ne-N-acetyllactosamine zisetshenziswe njengabamukeli kanye ne-uridine diphosphate (UDP) -galactose ne-cytidine monophosphate (CMP)-N-acetylneuraminic acid (NANA) njengabanikeli be-GalT-1 ne-ST-6, ngokulandelana.Imikhiqizo ye-enzymatic yayifakwe ilebula ku-situ ne-AA futhi yahlukaniswa nama-substrates kukholomu ye-TSKgel Amide 80 kusetshenziswa izimo zesigaba esivamile.Amayunithi e-enzyme anqunywa ezindaweni eziphakeme kakhulu ngokuqhathaniswa namazinga akhishwe ngesikhathi esifanayo i-Gal-β1-4GlcNAc kanye ne-NANA-α2-6 Gal-β1-4GlcNAc.I-Linearity (isikhathi nokugxilwa kwe-enzyme), ukunemba (i-intra- kanye ne-interassay) kanye nokuphindaphindeka kokuhlolwa kwasungulwa.Izivivinyo zitholakale ziwusizo ekuqapheni imisebenzi ye-enzyme ngesikhathi sokuhlukaniswa nokuhlanza.Ukuhlola bekuzwela kakhulu futhi kwenziwa ngokulingana noma okungcono kunezilinganiso ezisekelwe kushukela ezikhipha imisebe.Ifomethi yokuhlola ingase futhi isetshenziselwe ukulinganisa umsebenzi wokunye ukudluliselwa, inqobo nje uma abamukeli be-carbohydrate bequkethe isiphetho esincishisiwe sokulebula.Ukuhlolwa kwabamukeli be-glycoprotein kwasungulwa kusetshenziswa i-IgG.Indlela yephrofayili emfushane ye-HPLC yenzelwe ukuhlukaniswa kwe-IgG glycans (i-biantennary G0, G1, G2, mono- kanye ne-disialylated), eyenza ukuzimisela kwemisebenzi ye-GalT-1 kanye ne-ST-6 ngendlela esheshayo.Ngaphezu kwalokho, le ndlela yokwenza iphrofayela kufanele ibonakale iwusizo ekuqapheni izinguquko kuma-IgG glycans kuzilungiselelo zomtholampilo.
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