Imikhiqizo

Ngendlela ye-kinetic angiotensin-converting enzyme (ACE), isivali esisebenzayo nesilungile singama-80 mmol/L we-borate buffer ku-pH 8.2 (37 degrees C).Isigaba se-lag sitholwa ekuphenduleni, futhi ukufakwa kwe-5-min ye-substrate ne-plasma kuphakanyiswa ngaphambi kwesilinganiso se-kinetic.I-substrate, N--[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG), ukugxilisa ingqondo kukhuliswa ku-1.0 mmol/L futhi ubude begagasi bokulinganisa bungu-345 nm ukuze kuqinisekiswe umugqa wokulinganisa.Inqubo ehlongozwayo isebenzisa isilinganiso sevolumu ye-plasma-to-reagent engu-1:9.Ibanga lomugqa lokuhlolwa lifinyelela cishe ku-170 U/L, limele i-25% ye-substrate hydrolysis.Ukumuncwa kwe-FAPGG kunqunywa ngokulinganisa umehluko wokumunca phakathi kuka-1.0 mmol/L FAPGG kanye nezixazululo zomkhiqizo.Ukuthembeka kwe-wavelength kuhlolwa ngokuqaphela inani elilindelekile lokumunca lesisombululo se-FAPGG, futhi ukuchezuka kwe-1.0-nm kusuka ku-345 nm kushintsha ukumunca ngo-15.5%.Ukunemba kokuhlolwa kwe-ACE cishe ku-60 no-100 U/L kungu-3.5% no-2.4% phakathi kwenqwaba kanye no-2.9% no-2.6% phakathi kwenqwaba, ngokulandelana.Isikhathi sereferensi (amaphesenti angu-2.5 kuye kwangama-97.5) singu-41-139 U/L, futhi awukho umehluko phakathi kwamanani abesilisa nabesifazane.