Imikhiqizo

I-Acridine ester (NSP-SA-NHS) Cas199293-83-9 kanye nezinhlanganisela zayo ezihlobene zingamalebula e-chemiluminescent anenzuzo kakhulu ukuqina kwawo, umsebenzi nokuzwela kwawo okudlula amanye ama-radioisotopes.I-acridine esters ingasabela ngamaprotheni aqukethe amaqembu ayinhloko e-amino.Ngaphansi kwezimo eziyisisekelo, i-NHS ithathelwa indawo njengeqembu elishiyayo, futhi amaprotheni akha isibopho esizinzile se-amide ne-acridine ester.Ngemuva kokuthi ukusabela kuqediwe, usawoti we-acridinium owedlulele wasuswa ngekholomu ye-dessalting.

Amaprotheni anelebuli ye-Acridine awadingi i-enzymatic catalysis ukuze akhiphe ukukhanya phambi kwe-alkaline hydrogen peroxide.Umgomo othize okhipha ukukhanya ukuthi kusixazululo se-alkaline hydrogen peroxide, i-acridine ester ihlaselwa ama-ion e-hydrogen peroxide ukuze kukhiqizwe i-dioxyethane engazinzile ene-tension, ephinde iboliswe ibe yi-CO2 kanye ne-electronic acridone ejabule.Uma i-acridone ibuyela esimweni esiphansi, ikhipha ama-photon anobude obuphezulu bokumuncwa obungu-430 nm.Le nqubo ye-luminescence imfushane kakhulu (yonke inqubo ithatha imizuzwana engaphansi kwe-2), futhi uhlelo lokuqalisa kufanele lwengeze i-photometer yangaphakathi kanye nomtshina we-photon;ngaphezu kwalokho, lo mkhiqizo ungaphinda usebenzise isifundi se-microplate esinemisebenzi eminingi esine-autosampler yokuqoqwa kwedatha ye-luminescence.Amaprotheni, ama-peptide, amasosha omzimba, nama-nucleic acid konke kungalebula ngalo mkhiqizo.I-acridine esters ikhipha ukukhanya ngokushesha ngaphansi kwe-alkaline hydrogen peroxide, ngakho-ke izinhlanganisela ezilebulwe zingatholwa ngokuqoqa ama-photon.

Lo mkhiqizo usetshenziselwa ikakhulukazi: i-chemiluminescence kanye ne-immunoassay, ukuhlaziywa kwe-receptor, i-nucleic acid nokutholwa kwe-peptide nolunye ucwaningo.