I-ONPG CAS:369-07-3 98.0% Okumhlophe Okuncane Kuya Kucisha -Impushana Emhlophe
| Inombolo Yekhathalogi | XD90006 |
| I-CAS | 369-07-3 |
| Igama Lomkhiqizo | I-ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| I-Molecular Formula | I-C12H15NO8 |
| Isisindo samangqamuzana | 301.25 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 29400000 |
Ukucaciswa Komkhiqizo
| Ubumsulwa (HPLC) | Okuncane.98.0% |
| Ukubukeka | Impushana emhlophe ukuya emhlophe |
| Isixazululo(1% Emanzini) | Isixazululo esicacile, esingenambala ukuya kokuphuzi kancane |
| Okuqukethwe Amanzi(Karl Fisher) | Ubukhulu.0.5% |
| Ukujikeleza okucacile kokubona [α]D20(c=1, H2O) | - 65.0 ° C kuya -73.0 ° C |
Ingxoxo ngokuhlolwa kwe-ONPG (ukuhlolwa kwe-β-galactosidase)
Imibuzo ivame ukuphakanyiswa kamuva nje: 1. Kungani ukuhlolwa kwe-ONPG kungasetshenziswa ukuhlukanisa ukuvutshelwa kwe-lactose okulibaziseke?2. Kungani izinga likazwelonke lithi kuyadingeka ukusebenzisa i-3% sodium chloride trisaccharide iron (noma i-trisaccharide iron) ekuhlolweni kwe-ONPG?3. Ku-Vibrio parahaemolyticus, lapho kuhlolwa i-OPNG, kungani i-toluene kufanele yengezwe ngokudonsa phansi ngokuya ngokwendinganiso?Uyini umsebenzi?
Inkampani yethu ibuyekeze ulwazi oluningi, yalufingqa, futhi yabelana ngalo nawe ngezansi:
Isimiso: Igama lesiShayina le-ONPG lithi o-nitrobenzene-β-D-galactopyranoside.I-ONPG ingafakwa ku-hydrolyzed ibe yi-galactose kanye ne-o-nitrophenol ephuzi (ONP) nge-β-galactosidase, ngakho-ke umsebenzi we-β-galactosidase ungatholwa ngokushintsha kombala kwendawo yesiko.
I-Lactose ingushukela okudingeka ukuthi ama-microorganisms amaningi akwazi ukuyibona.I-metabolism yayo idinga ama-enzyme amabili, enye i-cell permease, i-lactose ingena kumaseli ngaphansi kwesenzo se-permease;enye i-β-galactosidase, eyenza i-lactose i-hydrolyze ibe i-galactose.I-Lactose ne-glucose.I-β-Galactosidase ingase futhi isebenze ngokuqondile ku-ONPG ukuze i-hydrolyze ibe i-galactose ne-o-nitrophenol ephuzi (ONP).Kungenziwa ngamahora angama-24, noma ngabe i-lactose ibambezelekile ukuvutshelwa.Ngakho-ke, ichaza imiphumela yokubuka yokukha isiko 1 kusuka ku-agar slant bese uyijova embuthanweni ogcwele ku-36 ° C ngo-1-3h no-24h.Uma i-β-galactosidase ikhiqizwa, izophenduka ibephuzi ngo-1-3h, uma ingekho i-enzyme enjalo, ngeke ishintshe umbala ku-24h.
Ngokwala ma-enzyme amabili angenhla, ama-microorganisms angahlukaniswa ngezigaba ezilandelayo:
I-1 lactose-fermenting (amahora angu-18-24) amagciwane ane-permease ne-β-galactosidase P + G +;
2 Izivubelo ze-lactose ezibambezelekile (ezithatha isikhathi esingaphezu kwamahora angu-24) zintula i-permease kodwa zine-galactosidase: P- G+.
3 Izivundisi ezingezona i-lactose ezintula kokubili i-permease ne-galactosidase: P- G-.
Ukuhlolwa kwe-ONPG kungasetshenziswa ukuhlukanisa amagciwane afaka i-lactose-lag-fermenting (P-G+) kubhaktheriya ye-lactose engabilisi (PG-), njengale:
1 Hlukanisa ukuvutshelwa kwe-lactose sekwephuzile (P- G+) ku-non-lactose fermenters (P- G-).
(a) I-Citrobacter (+) kanye ne-Salmonella arizonae (+) evela ku-Salmonella (-).
(b) I-Escherichia coli (+) evela ku-Shigella sonnei (-).
Kungani ukuhlolwa kwe-ONPG kwenziwa kusetshenziswa isiko lobusuku obubodwa ku-3% sodium chloride ferric trisaccharide (iron trisaccharide)?Inkampani yethu iye yabonisana ngolwazi oluningi, kodwa asikho isitatimende esicacile.Kuwebhusayithi ye-FDA kuphela, kubhaliwe ukuthi "Faka amasiko azohlolwa kuma-slants e-agar iron agar kathathu futhi afukamele amahora angu-18 ku-37°C (noma elinye izinga lokushisa elifanele, uma kudingekile) . Izakhi (noma ezinye) izikwele ze-agar eziqukethe u-1.0 I-% lactose ingasetshenziswa."kusho: amabhaktheriya okuhlola afakwe ku-iron trisaccharide medium futhi akhuliswe ku-37 ° C amahora angu-18.Ama-agar slants (noma okunye) okuphakathi okuqukethe u-1% we-lactose nawo ayamukeleka.Ngakho-ke, kucatshangwa ukuthi i-trisaccharide iron medium iqukethe i-lactose.Ngemva kokukhula ngobusuku obubodwa, amagciwane akhiqize i-β-galactosidase esebenzayo esebenzayo.Ngokusebenzisa amagciwane anjalo, i-ONPG ingabola nge-β-galactosidase ngokushesha.Isenzakalo sokuhlola siyashesha futhi sibonakala kangcono.Ngaphezu kwalokho, ukungezwa kwe-toluene kanye nokugeza kwamanzi imizuzu emi-5 konke kwenzelwa ukukhulula ngokugcwele i-β-galactosidase.
