I-Phosphatase, i-alkaline CAS:9001-78-9 emhlophe, empunga emhlophe noma i-peach monoclinic crystal noma impushana
| Inombolo Yekhathalogi | XD90379 |
| Igama Lomkhiqizo | I-Phosphatase, i-alkaline |
| I-CAS | 9001-78-9 |
| I-Molecular Formula | I-C21H36N8O6 |
| Isisindo samangqamuzana | 496.57 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 35079090 |
Ukucaciswa Komkhiqizo
| Isivivinyo | 99% |
| Ukubukeka | emhlophe, empunga emhlophe noma e-peach monoclinic crystal noma impushana |
Nakuba i-fluoride yaziwa ngokugqugquzela ukwakheka kwamathambo, izinqubo eziyisisekelo aziqondwa ngokugcwele.Ucwaningo lwakamuva luye lwafaka phakathi indlela ye-Wnt/β-catenin njengendlela enkulu yokubonisa i-bone biology.Ucwaningo lwethu lwangaphambilini lwagqamisa indima engaba khona ye-canonical Wnt pathway ekwakhekeni kwamathambo amagundane ahlala edalulwe yi-fluoride, kodwa indlela yokusebenza ihlala ingacacile.Ucwaningo lwamanje lunqume ukubandakanyeka kokubonisa i-Wnt/β-catenin ekuhlukaniseni i-osteoblastic eyenziwe nge-fluoride.Sisebenzisa ama-osteoblasts amagundane ayinhloko, sibonise ukuthi i-fluoride ikhuthaza kakhulu ukwanda kwe-osteoblasts kanye nenkulumo ye-alkaline phosphate (ALP) kanye namazinga wokuvezwa kwe-mRNA wezimpawu zokuhlukanisa amathambo, okuhlanganisa uhlobo I-collagen (COL1A1), i-ALP ne-osteonectin.Siphinde sathola ama-phosphorylations enziwe nge-fluoride ku-serine 473 ye-Akt kanye ne-serine 9 ye-glycogen synthase kinase-3β (GSK3β), okuholele ekuvimbeni kwe-GSK-3β futhi kamuva nokuhlaziywa kwe-nuclear ye-β-catenin, njengoba kuboniswa yi-Western blot kanye ne-immunofluoresce .Ngaphezu kwalokho, i-fluoride iphinde yaheha ukuvezwa kofuzo oluhloselwe i-Wnt-runt-related transcription factor 2 (Runx2).Okubalulekile, umphumela omuhle we-fluoride kumsebenzi we-ALP kanye nezinkulumo ze-mRNA ze-COL1A1, ALP, i-osteonection kanye ne-Runx2 yaqedwa yi-DKK-1, i-blocker ye-Wnt/β-catenin receptor.Kuhlanganiswe ndawonye, lokhu okutholakele kusikisela ukuthi i-fluoride ikhuthaza ukuhlukaniswa kwe-osteoblastic ngokusebenzisa ukusebenza okuncike ku-Akt- kanye ne-GSK-3β-yendlela yokusayina ye-Wnt/β-catenin kuma-osteoblasts amagundane ayinhloko.Okutholakele kwethu kuhlinzeka ngemininingwane emisha ezindleleni zesenzo se-fluoride ku-osteoblastogenesis.
