Imikhiqizo

I-Borna disease virus (BDV) iyigciwane le-noncytolytic, neurotrophic elithelela izinhlobonhlobo zezilwane ezinomgogodla, okuhlanganisa zonke izilwane ezinegazi elifudumele futhi mhlawumbe nabantu.Nakuba ukutheleleka kwe-BDV kucatshangwa ukuthi kubangele ukuphazamiseka kwezinzwa, ubufakazi bokuba khona kwegciwane ezicutshini noma egazini leziguli ezigula ngengqondo bulinganiselwe, mhlawumbe ngenxa yokuzwela okuphansi kwezindlela zokuthola.Lapha, indlela elula yokuthwebula i-BDV yenziwe kusetshenziswa ubuhlalu kazibuthe obumbozwe nge-anionic polymer, i-poly(methyl vinyl ether-maleic anhydrate).Ubuhlalu bebufakwe i-lysate obusuka kumaseli atheleleke nge-BDV, bese buhlukaniswa nonamandla amakhulu ngokufaka inkundla kazibuthe bese bugezwa.Ukukhangiswa kwe-BDV ngobuhlalu kwaqinisekiswa ukuhlanekezela kwe-reverse transcription-polymerase chain kanye ne-Western blotting, ebonisa ukuba khona kwe-phosphoprotein (P), i-nucleoprotein (N), kanye ne-viral genome ye-BDV ebuhlalu obufukanyelwe.Le ndlela yokuthwebula ingase ibe nomthelela ekutholakaleni okuthuthukisiwe kwe-BDV.