Imikhiqizo

Ama-Multitarget compounds aya ngokuya ephishekela ukwelashwa okuphumelelayo kwezifo eziyinkimbinkimbi.Lapha, sichaza idizayini kanye nokuhlanganiswa kwesigaba senoveli ye-shogaol-huprine hybrids, okuhloswe ngayo ukuthi ifinyelele imigomo eminingana ebalulekile ehilelekile kusifo i-Alzheimer's.Ama-hybrids ahlolwe ku-vitro ngomsebenzi wawo ovimbelayo ngokumelene ne-acetylcholinesterase yabantu kanye ne-butyrylcholinesterase nomsebenzi we-antioxidant (ABTS +, DPPH kanye ne-Folin-Ciocalteu assays), kanye namaseli e-Escherichia coli aqinile ngomsebenzi wawo we-Aβ42 kanye ne-tau anti-aggregating.Futhi, ukungena kwabo kobuchopho kuye kwahlolwa (i-PAMPA-BBB assay).Ngisho noma ama-hybrids engewona ama-AChE inhibitors noma ama-antioxidant anamandla njenge-huprine Y kanye ne- [4] -shogaol yomzali, ngokulandelana, asabonisa imisebenzi enamandla ye-anticholinesterase kanye ne-antioxidant futhi enamandla kakhulu i-Aβ42 kanye ne-tau anti-aggregating agents kune- izinhlanganisela zomzali.Sekukonke, ama-shogaol-huprine hybrids avela njengokuhola okuthakaselayo kobuchopho okuphemelezeka okuningi kwe-anti-Alzheimer.

Izingcaphuno ze-polarity ehlukene ezitholwe ezingxenyeni ezihlukahlukene zezitshalo (impande, iqabunga, imbali nesithelo) ze-Seseli rigidum zacutshungulwa ngokuhlolwa okuhlukile kwe-antioxidant: Umsebenzi we-DPPH kanye ne-ABTS wokuqothula okunamandla, ngokunciphisa ngokuphelele indlela yamandla kanye nokuqukethwe okuphelele kwama-flavonoid nama-polyphenols. .Amafutha abalulekile azo zonke izingxenye zezitshalo abonise izici ezibuthakathaka ze-antioxidant.Ibanga lokuhlushwa elivinjiwe lezinto ezikhishiwe ezihloliwe, ngokumelene nebhaktheriya i-Escherichia coli, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Bacillus cereus, nesikhunta i-Candida albicans kanye ne-Aspergillus niger yayingu-0.01-1.50 mg/mL kanye ne-microbicidal 300 mg/mL0.0.Ekusebenzelaneni ne-cholinesterase, wonke amafutha abalulekile abonakale esebenza njengama-inhibitors.Iphesenti eliphakeme kakhulu lokuvinjelwa uma liqhathaniswa ne-cholinesterase yomuntu nehhashi liboniswe ngamafutha abalulekile ezimpande (38.20% no-48.30%, ngokulandelana) phakathi kwamafutha, kanye ne-root hexane extract (40.56% kanye no-50.65% ngokulandelana).Amafutha abalulekile kanye nezingxenye eziguquguqukayo zazo zonke izingxenye zezitshalo zihlonzwe yi-GC, i-GC-MS kanye ne-headspace/GC-MS.Ukuhlaziywa kwezibalo kokuhlanganiswa kwemiphumela kubonise ukuthi ukwakheka kwamafutha abalulekile kwempande kuhluke kakhulu kumafutha abalulekile wezinye izingxenye zesitshalo.Kucatshangelwa yonke imisebenzi efundiwe, ukukhishwa kwempande kwe-hexane kubonise izici ezingcono kakhulu zizonke.Ngokusebenza okuphezulu kwe-chromatography yoketshezi ehlanganiswe nokucaca okuphezulu kwe-mass spectrometry, izakhi ezingu-30 ezichichima kakhulu zikhonjwe ekukhishweni kwe-polarity ehlukile.Ukuba khona kwezakhi ezihlonziwe kwaxhunyaniswa nemisebenzi ethile yebhayoloji eqashiwe, ngaleyo ndlela kuqokwa izinhlanganisela okungenzeka zibe nesibopho somsebenzi ngamunye obonisiwe.