Imikhiqizo

Ukukhishwa kwe-Chemiluminescence (CL) okuvela kuhlelo lwe-luminol-tetrachloroaurate ([AuCl(4)](-)) okufundwe lapho kukhona ushukela we-monosaccharide ofana ne-glucose ne-fructose kwaphenywa ku-microfluidic chip eyakhiwe inqubo ye-lithography ethambile.Ukukhishwa kwe-CL okuvela ohlelweni lwe-luminol-[AuCl(4)](-) ku-430 nm kwaqiniswa ngokuphawulekayo umsebenzi we-catalytic we-glucose ne-fructose ekamelweni lokushisa.Ngaphansi kwezimo ezithuthukisiwe, ukushuba kokukhipha kwe-CL kwesistimu kutholwe kuhlobene ngokuhambisana nokugcwala koshukela.Ngokusekelwe kulokhu kuqashelwa, ukunqunywa okungezona kwe-nenzymatic koshukela ophelele (i-glucose, i-fructose, noma i-hydrolyzable sucrose) kwenziwa ngendlela yokuhlaziya esheshayo nebucayi.Imiphumela yembule ukuthi i-linearity isukela ku-9 kuya ku-1,750 μM ye-glucose kanye ne-80 kuya ku-1,750 μM ye-fructose, enomkhawulo wokutholwa kwe-0.65 no-0.69 μM, ngokulandelana.Ukuchezuka okujwayelekile okuhlotshaniswayo okunqunywe ku-250 μM ngokusekelwe emijoweni ephindaphindiwe eyisithupha kwakungu-1.13 no-1.15% we-gluc ose ne-fructose, ngokulandelana.Indlela ethuthukisiwe isetshenziswe ngempumelelo ukuze kunqunywe inani likashukela eliphelele ekudleni naseziphuzweni.