I-Streptomycin sulfate CAS:3810-74-0 Impuphu emhlophe
| Inombolo Yekhathalogi | XD90361 |
| Igama Lomkhiqizo | I-Streptomycin sulfate |
| I-CAS | 3810-74-0 |
| I-Molecular Formula | (C21H39N7O12)2·3H2SO4 |
| Isisindo samangqamuzana | 1457.39 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 29412080 |
Ukucaciswa Komkhiqizo
| Ibanga | USP36/EP8.0 |
| pH | 4.5-7.0 |
| Ukulahlekelwa Ekumisweni | ≤7.0% |
| Isivivinyo | ≥720IU/MG |
| I-sulfate | 18.0~ 21.5% |
| Ukubukeka | Impushana emhlophe noma ecishe ibe mhlophe, incibilika kakhulu emanzini, cishe ayincibiliki ku-ethanol. |
| I-Methanol | ≥0.3% |
| I-Streptomycin B | ≤3.0% |
| Ukuhlolwa kombala | ≥90.0% |
| I-Sulfuric Acid | ≤1.0% |
| Okuqukethwe kwe-sodium sulphite okugxilile | ≤0.4% |
Nakuba i-etiology ye-intervertebral disc degeneration ingaqondakali kahle, enye indlela yokuvimbela le nqubo ingase ibe ukuvimbela i-apoptosis.Ocwaningweni lwamanje, imiphumela ye-anti-apoptotic ye-carboxymethylated chitosan (CMCS) kumaseli e-nucleus pulposus (NP) iphenywe ngenhloso yokuthuthukisa ukusinda kwe-disc cell.Amaseli e-Rat NP ahlukaniswa futhi akhuliswa ku-vitro, futhi i-hydrogen peroxide (H2O2) yasetshenziswa ukwakha imodeli ye-NP cell apoptosis.Ukusebenza kweseli kwahlolwa ngokuhlolwa kwekhithi yokubala kwamaseli-8.Isilinganiso samaseli e-apoptotic sahlolwa yi-annexin V‑fluorescein isothiocyanate (FITC) kanye ne-propidium iodide (PI) ukuhlaziywa okuphindwe kabili kwamabala, futhi i-morphology yabonwa ngu-Hoechst 33342 staining.Amandla olwelwesi lwe-mitochondrial lwamaseli e-NP ahlolwe nge-rhodamine 123 fluorescence staining.I-Reverse transcription (RT)‑quantitative polymerase chain reaction (qPCR) yenzelwa ukukala amazinga e-mRNA e-inducible nitric oxide synthase (iNOS), caspase‑3, B-cell lymphoma (Bcl)‑2, uhlobo lwe-II collagen ne-aggrecan.Ukuhlaziywa kwe-blot yaseNtshonalanga kwenziwa ukuze kutholwe amazinga amaprotheni e-iNOS ne-Bcl-2.Imiphumela ye-annexin V-FITC/PI kanye ne-Hoechst 33342 ibonise ukuthi i-CMCS ikwazile ukuvimbela amaseli e-NP kusukela ku-apoptosis ngendlela encike kumthamo.I-Rhodamine 123 staining icacise ukuthi i-CMCS yehlise ukonakala kwamandla olwelwesi lwe-mitochondrial kumaseli e-NP alashwe nge-H2O2.Umsebenzi owehlisiwe we-caspase‑3 kanye nomsebenzi okhulisiwe we-Bcl-2 kutholwe kumaseli e-NP alashwe nge-CMCS nge-RT-qPCR kanye nokuhlaziywa kwe-western blot.I-CMCS iphinde yakhuthaza ukwanda nokukhiqizwa kohlobo lwe-collagen lwe-II kanye ne-aggrecan kumaseli e-NP alashwe nge-H2O2.I-CMCS iboniswe ukuthi iphumelele ekuvimbeleni i-apoptotic cell death in vitro, ebonisa izinzuzo ezingase zibe khona zale ndlela yokwelapha ekulawuleni ukuwohloka kwe-disc.
