Imikhiqizo

I-Flow cytometry iyindlela eyinhloso yokuhlola ukuzwela kwe-antimalarial ku-vitro enamandla akhulayo okusetshenziswa kumasayithi enkambu.Sihlole ukuthambekela kwe-in vitro emithini elwa nomalaleveva eyisikhombisa ye-P. falciparum entsha engu-40 ehlukanisiwe ngendlela ye-flow cytometry (FCM), i-ELISA esekelwe ku-colorimetric ye-LDH : DELI), kanye nokuhlaziywa okujwayelekile kwesilayidi se-microscopic sokukhula.Ku-FCM, ukuhlola okungu-184/280 (66%) kuhlangabezane nemibandela yokwamukelwa kohlaziyo, uma kuqhathaniswa no-166/280 (59%) we-DELI.Kube nesivumelwano esihle phakathi kwe-FCM ne-microscopy, kuyilapho i-DELI ijwayele ukukhiqiza ama-inhibition constants aphezulu angamahhafu aphezulu (IC50s) kune-FCM, ngokuchema sekukonke kwe-2.2-fold (ukuqhathanisa kwe-Bland-Altman).Amanani e-artesunate ne-dihydroartemisinin athinteke kakhulu.Ukwenyuka okuxakayo kwesignali ekugxilweni okuphezulu kakhulu kwe-mefloquine nezinhlanganisela ezihlobene kwakumakwe kakhulu ngokuhlolwa kwe-DELI, okuphakamisa ukuthi imiphumela engeyona eqondiwe ekukhiqizweni kwe-LDH ingaba nesibopho.Ukulahleka kwesignali ye-FCM ngenxa yokuhlaselwa kabusha noma ukukhula kancane kubonwe enanini elincane lamasampuli.Le miphumela inweba umsebenzi wangaphambilini wokusetshenziswa kwe-flow cytometry ukuze kutholwe ukuthambekela kokulwa nomalaleveva ngokuya ngenani lamasampula, uhla lwezidakamizwa, kanye nokuqhathaniswa nezinye izindlela.