Imikhiqizo

I-Cysteine ​​(Cys) idlala indima ebalulekile ezinqubweni eziningi zomzimba zamaseli e-eukaryotic futhi ukutholakala kwayo kumaseli kubaluleke kakhulu.Kodwa-ke, i-glutathione (GSH), i-homocysteine, i-N-acetyl-L-cysteine ​​kanye namanye ama-thiol aphazamisa kakhulu ukutholwa kwamaCys.Ikakhulukazi, i-GSH iphazamisa kakhulu ukutholwa kwama-Cys eselula (30-200 μM) ngenxa yokugxila kwayo okuphezulu kwe-intracellular (1-10 mM).Kulo msebenzi, kwethulwa i-off-on fluorescent probe (HOTA) ukuze kutholwe i-Cys.Le probe inokubili ukuzwela okuhle kakhulu kanye nokukhetha okwanelisayo kokutholwa kwe-Cys yeselula: ngokwengezwa kwama-Cys angu-200 μM, amandla e-fluorescence ophenyo (10 μM) athuthukiswe ngokuphindwe ka-117 futhi umkhawulo wokutholwa ubalwe waba ngu-13.47 μM, ophansi. kune-Cys concentration yeselula;i-probe iphinde yathola ngokukhetha i-30–200 μM i-cysteine ​​phezu kuka-1–10 mM glutathione.Ngenxa yalokho, ukuhlolwa kwesithombe seseli kwenziwa nge-HOTA yophenyo.Ngaphezu kwalokho, imiphumela yokuhlolwa kwe-thiol-blocki ng kanye ne-GSH synthesis inhibiting test iqinisekisile ukuthi ukukhishwa kwe-intracellular ngokuyinhloko kuvela ekusebenzelaneni phakathi kwe-Cys ne-HOTA.