Imikhiqizo

Isingeniso:I-Tris-Hydroxymethylaminomethane hydrochloride ibizwa ngokuthi i-Tris-HCl.I-Tris-HCl buffer (i-Tris-HCl buffer) ayisetshenziswa kabanzi nje kuphela njenge-solvent yama-nucleic acid namaprotheni, kodwa futhi inokusetshenziswa okuningi okubalulekile.

Isicelo:I-Tris-HCl isetshenziselwe ukukhula kwekristalu yamaprotheni ezimweni ezihlukile ze-pH.Amandla e-ionic aphansi webhafa ye-Tris-HCL angasetshenziselwa ukwakheka kwemicu emaphakathi ye-lamin kuma-C. elegans.I-Tris-HCl futhi ingenye yezingxenye eziyinhloko zama-protein electrophoresis buffers.Ngaphezu kwalokho, i-Tris-HCL iphinde ibe yindawo emaphakathi yokulungiswa kwama-surfactants, ama-vulcanization accelerators nezinye izidakamizwa ze-Chemicalbook.I-Tris-HCl nayo yasetshenziswa njengezinga le-titration.

Ukulungiswa kwesixazululo sebhafa:thatha i-tris(hydroxymethyl)aminomethane hydrochloride njenge-solute, thatha amanzi akhishwe yi-deionized njengesincibilikisi, bese usebenzisa isixazululo samanzi se-HCl esinengxube engu-0.1mol/L ukulungisa inani le-pH ukuze ulungise isixazululo se-trimethylolamine esinokuhlangana kwe-6.057g/L kanye ne-pH=8.8.Isixazululo sebhafa se-Hydroxymethylaminomethane hydrochloride (ibhafa ye-Tris-HCl).

Izakhiwo Zekhemikhali:Amakristalu amhlophe.

Ukusetshenziswa:I-pH yawo wonke amabhafa izinga lokushisa nokugxilisa ingqondo kuncike.Kubhafa ye-Tris, i-pH inyuka cishe ngamayunithi angu-0.03 ngokunyuka okungu-1 °C kwezinga lokushisa futhi yehla ngamayunithi angu-0.03-0.05 ngokuhlanjululwa okuphindwe ka-10 ngakunye.

Isicelo:yebhafa yebhayoloji

Isebenzisa:esetshenziswa njengeziphakathi ku-organic synthesis