I-X-GAL CAS:7240-90-6 98% I-Crystalline Powder emhlophe ukuya kokumhlophe
| Inombolo Yekhathalogi | XD90008 |
| Igama Lomkhiqizo | I-X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| I-CAS | 7240-90-6 |
| I-Molecular Formula | I-C14H15BrClNO6 |
| Isisindo samangqamuzana | 408.63 |
| Imininingwane Yesitoreji | -2 kuya ku-6 ° C |
| Ikhodi Yentela Evumelanisiwe | 29400000 |
Ukucaciswa Komkhiqizo
| Ukubukeka Kwesixazululo | Isixazululo esicacile, esingenambala ukuya kokuphuzi ngokukhanyayo (50mg/ml ku-DMF:MeOH, 1:1) |
| Ukujikeleza okuqondile okubonakalayo | -61.5 +/- 1 |
| Ukubukeka | Impushana yekristalu emhlophe ukuya kokumhlophe |
| I-Purity HPLC | iminithi 99% |
| Ukunyibilika (5 % ku-DMF) | I-Soluble (5% w/v,DMF) |
| Amanzi KF | ubuningi 1% |
| I-Assay (HPLC ku-Anhydrous Basis) | iminithi 98% w/w |
Ukusetshenziswa kwe-X-gal
I-X-gal (futhi efushanisiwe i-BCIG ye-5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) iyinhlanganisela yemvelo ehlanganisa i-galactose exhunywe ku-indole efakwe esikhundleni.Inhlanganisela yahlanganiswa ngu-Jerome Horwitz nabahlanganyeli ngo-1964.Igama lekhemikhali elisemthethweni livamise ukufushaniswa ukuze libe nemishwana enembe kancane kodwa futhi enzima kakhulu njenge-bromochloroindoxyl galactoside.I-X evela ku-indoxyl ingase ibe umthombo we-X ekufinyezweni kwe-X-gal.I-X-gal ivamise ukusetshenziswa kubhayoloji yamangqamuzana ukuhlola ukuba khona kwe-enzyme, i-β-galactosidase, endaweni yethagethi yayo evamile, i-β-galactoside.Ibuye isetshenziselwe ukuthola umsebenzi wale enzyme ku-histochemistry kanye ne-bacteriology.I-X-gal ingenye yama-indoxyl glycosides amaningi nama-ester akhiqiza inhlanganisela eluhlaza okwesibhakabhaka engancibiliki efana nodayi we-indigo ngenxa ye-enzyme-catalyzed hydrolysis.
I-X-gal iyi-analog ye-lactose, ngakho-ke ingase yenziwe i-hydrolyzed yi-enzyme ye-β-galactosidase ehlukanisa ibhondi ye-β-glycosidic ku-D-lactose.I-X-gal, lapho iqhekezwa yi-β-galactosidase, ikhiqiza i-galactose kanye ne-5-bromo- 4-chloro-3-hydroxyindole - 1. Le yokugcina ibe isincisha ngokuzenzakalelayo futhi ifakwe i-oxidized ibe yi-5,5'-dibromo-4,4'-dichloro -indigo - 2, umkhiqizo oluhlaza okwesibhakabhaka kakhulu ongancibiliki.I-X-gal ngokwayo ayinambala, ngakho-ke ukuba khona komkhiqizo onombala ohlaza okwesibhakabhaka kungase kusetshenziswe njengokuhlola ukuba khona kwe-β-galactosidase esebenzayo.Lokhu futhi kuvumela ukuthi i-β-galactosidase yebhaktheriya (ebizwa ngokuthi i-lacZ ) isetshenziswe njengentatheli ezinhlelweni ezihlukahlukene.
Ekuhlaziyweni okuyingxubevange emibili, i-β-galactosidase ingasetshenziswa njengentatheli ukuhlonza amaprotheni asebenzisanayo.Ngale ndlela, imitapo yolwazi yofuzo ingase ihlolelwe ukusebenzisana kwamaprotheni kusetshenziswa imvubelo noma uhlelo lwebhaktheriya.Lapho kunokusebenzelana okuyimpumelelo phakathi kwamaprotheni ahlolwayo, kuzophumela ekubopheni isizinda sokwenza kusebenze kumthuthukisi.Uma umgqugquzeli exhunyaniswa nesakhi sofuzo se-lacZ, ukukhiqizwa kwe-β-galactosidase, okuholela ekwakhekeni kwamakholomu anemibala eluhlaza okwesibhakabhaka phambi kwe-X-gal, ngakho-ke kuzobonisa ukusebenzisana okuphumelelayo phakathi kwamaprotheni.Le nqubo ingase ikhawulelwe ekuhloleni amalabhulali anosayizi ongaphansi kuka-106. Ukuqhekeka okuyimpumelelo kwe-X-gal nakho kudala iphunga elibi ngokuphawulekayo ngenxa yokuguquguquka kwe-indole.
Njengoba i-X-gal ngokwayo ingenambala, ukuba khona komkhiqizo onombala ohlaza okwesibhakabhaka kungase kusetshenziswe njengokuhlola ukuba khona kwe-β-galactosidase esebenzayo.
Lokhu kukhonjwa okulula kwe-enzyme esebenzayo kuvumela isakhi sofuzo se-βgalactosidase (ufuzo lwe-lacZ) ukuthi sisetshenziswe njengofuzo lwentatheli ezinhlelweni ezahlukahlukene.
