Imikhiqizo

1.I-D-2-hydroxybutanoate esebenzayo ngokubonakalayo iyibhulokhi yokwakha ebalulekile emaphakathi emithini kanye ne-biodegradable poly(2-hydroxybutanoate).Ukulungiswa kwe-Kinetic kwe-racemic 2-hydroxybutanoate kungase kube enye indlela eluhlaza nefiselekayo ekukhiqizeni kwe-D-2-hydroxybutanoate.Kulo msebenzi, i-D-2-hydroxybutanoate ekugxilweni okuphezulu (0.197 M) kanye nokweqile kwe-enantiomeric (99.1%) kwakhiqizwa i-NAD-independent L-lactate dehydrogenase (L-iLDH) equkethe i-biocatalyst.I-2-Oxobutanoate, enye i-intermediate ebalulekile, yenziwe ngokuhlanganyela ekugxilweni okuphezulu (0.193 M).Ukusebenzisa inqubo elula yokushintshanisa i-ion nge-macroporous anion exchange resin D301, i-D-2-hydroxybutanoate yahlukaniswa nesistimu ye-biotransformation ngokuthola okuphezulu kwe-84.7%.

2.Ukwenziwa kwe-asymmetric ye-amino acid engeyona eyemvelo kwaboniswa i-omega-transaminase evela ku-Vibrio fluvialis JS17.I-L-2-Aminobutyric acid yahlanganiswa kusuka ku-2-oxobutyric acid kanye ne-benzylamine ene-enantiomeric ngokweqile engaphezu kuka-99%.Ukusabela kwabonisa ukuvinjelwa okunzima komkhiqizo nge-benzaldehyde, okwanqotshwa ngokusebenzisa isistimu yokusabela kwe-biphasic ukususa umkhiqizo ovimbelayo esigabeni esimanzi.Ekuphenduleni okujwayelekile kwe-biphasic (50 mM 2-oxobutyric acid, 70 mM benzylamine kanye ne-enzyme ehlanzekile engu-2.64 U/ml) kusetshenziswa i-hexane njenge-extractant, ukuguqulwa kwe-2-oxobutyric acid kufinyelele ku-96% emahoreni amahlanu kuyilapho ukuguqulwa okungama-39% kuphela kutholwe ngaphandle. ukukhishwa komkhiqizo.