4-Aminophthalhydrazide AMPPD Cas:3682-14-2 Okuphuzi okukhanyayo kuya ku-amber ukuya kumpushana oluhlaza okumnyama kuya kuqhubu
| Inombolo Yekhathalogi | XD90156 |
| Igama Lomkhiqizo | 4-Aminophthalhydrazide |
| I-CAS | 3682-14-2 |
| I-Molecular Formula | I-C8H7N3O2 |
| Isisindo samangqamuzana | 177.16 |
| Imininingwane Yesitoreji | Gcina kwa-RT |
Ukucaciswa Komkhiqizo
| Ukubukeka | Okuphuzi ngokukhanyayo ukuya ku-amber ukuya kumpushana oluhlaza okotshani kuya kugaqa |
| U-Asay | ≥98.0% |
| Ukuminyana | 1.433 |
| Iphoyinti lokuncibilika | 300 ºC |
| Iphuzu elibilayo | 633°C ku-760 mmHg |
| Iphoyinti le-Flash | 336.7°C |
| Ukuncibilika | Incibilika ku-ammonium hydroxide, sodium carbonate noma esinye isisekelo. |
1.Sihlaziye futhi siqhathanisa umphumela wama-H1-antihistamines amahlanu ekuqhumeni kwe-oxidative evuselelwe ezingeni le-extra- kanye ne-intracellular ye-polymorphonuclear leukocyte yabantu ehlukanisiwe futhi evuselelwe.Ukuqhuma kwe-oxidative yama-neutrophils abantu abangabodwa kwafundwa ngokusebenzisa i-luminol ne-isoluminol ethuthukisiwe i-chemiluminescence elandelayo. I-rank order of potency ye-H1-antihistamines ukunciphisa i-chemiluminescence yahlolwa ngaphandle kwe-extracellularly: dithiaden> loratadine> chlorpheniramine> brompheniramine> pheniramine kanye nendawo ye-intracellular: i-loratadine> i-dithiaden. - kanye nendawo ye-intracellular yama-neutrophils avuselelwe abantu.
2.Amasistimu e-Chemiluminescence athuthukiswa yi-isoluminol noma i-luminol ngokuhambisana ne-peroxidase izindlela ezibucayi zokutholwa kwezinhlobo ze-oxygen esebenzayo (ROS) ekhiqizwa i-phagocyte NADPH oxidase.Ama-substrates amabili akhulisayo ayafana kakhulu ngokwesakhiwo, ahluka kuphela endaweni yeqembu lama-amino eringini enamakha yama-molecule.Lo mehluko wenza i-isoluminol ibe i-molecule encane ye-lipophilic engangeneki kancane kulwelwesi lwebhayoloji.Ukusetshenziswa kwe-isoluminol ngenxa yalokho kukhawulelwe ezifundweni eziphathelene nokugcinwa kwe-oksijini metabolites.Kulolu cwaningo sibonisa ukuthi ama-peptide okwenziwa atholakala esizindeni se-N-terminal se-calcium-regulated protein annexin AI aphazamisa ukutholwa kwama-radicals ku-isoluminol-amplified, kodwa hhayi ohlelweni lwe-luminol-amplified.Ama-peptide atholakala ku-annexin AI anciphisa ukuphuma kokukhanya nge-isoluminol ejatshuliswe yi-superoxide ne-horseradish peroxidase (HRP) ku-formyl-methionyl-leucyl-phenylalanine- kanye namaseli ami avuselelwe i-ristate acetate, kanye ne-hydrogen peroxide ne-HRP.Indlela eqondile yokuvimbela akwaziwa.Imiphumela ethulwe ngokuqinile iphakamisa ukuthi impendulo encishisiwe yeselula etholwe nge-isoluminol-amplified chemiluminescence kufanele iqinisekiswe ngenye indlela yokunquma ukukhishwa kwama-anion e-superoxide ne-hydrogen peroxide.
