Amaprotheni okucubungula isignali ye-PII asakazwa kabanzi kuma-prokaryotes nasezitshalweni lapho alawula inqwaba yokusabela kwe-anabolic.Ukukhiqizwa ngokweqile kwama-metabolites kudinga ukuphumuza amasekhethi aqinile okulawula amaselula.Lapha sibonisa ukuthi ukuguqulwa kwephuzu elilodwa kuphrotheni ekhombisa izimpawu ze-PII kusuka ku-cyanobacterium Synechocystis sp.I-PCC 6803 yanele ukuvula indlela ye-arginine ebangela ukunqwabelana kwe-biopolymer cyanophycin (multi-L-arginyl-poly-L-aspartate).Lo mkhiqizo unentshisekelo ye-biotechnological njengomthombo wama-amino acid kanye ne-polyaspartic acid.Lo msebenzi uyisibonelo sendlela entsha yobunjiniyela bendlela ngokuklama amaprotheni amasignali e-PII aklanywe ngokwezifiso.Lapha, i-Synechocystis sp eyakhiwe.I-PCC6803 uhlobo olunokuguqulwa kwe-PII-I86N okuqoqwe ngaphezulu kwe-arginine ngokusebenzisa ukwakheka kusebenze kwe-enzyme engukhiye i-N-acetylglutamate kinase (NAGK).Kuhlobo lobunjiniyela lwe-BW86, ku-vivo umsebenzi we-NAGK uye wanda kakhulu futhi waholela kokuqukethwe okuphezulu kwe-arginine okuphindwe kashumi kunasendle.Ngenxa yalokho, uhlobo lwe-BW86 luqongelele kufika ku-57 % i-cyanophycin ngeseli eyomile ngaphansi kwezimo ezihloliwe, okuyisivuno esiphezulu se-cyanophycin esibikwe kuze kube manje.I-Strain BW86 ikhiqize i-cyanophycin ebangeni lesisindo samangqamuzana angama-25 kuya ku->100 kDa;uhlobo lwasendle lukhiqize i-polymer ebangeni elingama-30 kuya ku->100 kDa. Isivuno esiphezulu nesisindo se-molecular mass ye-cyanophycin ekhiqizwa uhlobo lwe-BW86 kanye nezidingo eziphansi zomsoco we-cyanobacteria kuyenza ibe yindlela ethembisayo yokukhiqizwa kwe-biotechnological ye-cyanophycin.Lolu cwaningo luphinde lukhombise ukuba nokwenzeka kobunjiniyela bezindlela ze-metabolic kusetshenziswa iphrotheni ekhombisa izimpawu ze-PII, eyenzeka kumabhaktheriya amaningi.
