Imikhiqizo

I-aptasensor esebenzayo yasungulwa lapho i-graphene oxide (GO) yaqashwa khona njengenkomba yakho kokubili i-electrochemical impedance spectroscopy kanye nokukhiqizwa kwesignali ye-electrochemiluminescence (ECL).I-aptasensor yaqanjwa ngokuzihlanganisa ngokwayo i-ECL probe ye-thiolated adenosine triphosphate binding aptamer (ABA) emakwe ngokuphuma kokuphuma kwe-Ru complex (Ru(bpy)3(2+) phezu kwe-nanoparticle (AuNP) eguquliwe yekhabhoni eyingilazi. i-electrode (GCE).I-ABA enganyakazi ku-AuNP eshintshiwe ye-GCE ingakhangisa kakhulu i-GO ngenxa yokusebenzisana okuqinile kwe-π-π phakathi kwe-ABA ne-graphene oxide;Ukucinywa kwe-ECL kwe-Ru complex bese kwenzeka ngenxa yokudluliswa kwamandla nokudluliswa kwe-electron, nokwanda okukhulu kokumelana nokudluliswa kwe-electron (Ret) kwe-electrode.Ngenkathi ikhona i-adenosine triphosphate (ATP) ehlosiwe, i-ABA ikhetha ukwenza i-ABA-ATP bioaffinity complexes, enobudlelwane obubuthakathaka ne-graphene oxide futhi igcina i-graphene oxide ikude nendawo ye-electrode, ngaleyo ndlela ivumele ukuthuthukiswa kwesignali ye-ECL, futhi ngokuhambisana nokuncipha kwe-Ret.Ngenxa yokusebenza kahle kokucisha kwe-ECL, ukwakheka okuyingqayizivele, kanye nezakhiwo zikagesi ze-graphene oxide, ukuqina kwe-Ret ne-ECL ngokuqhathaniswa ne-logarithm yokugxilisa ingqondo ye-ATP bekumugqa ebangeni elibanzi ukusuka ku-10 pM kuye ku-10 nM enomkhawulo wokutholwa ophansi kakhulu ongu-6.7 pM kuye ku-4.8 pM, ngokulandelana.I-aptasensor ehlongozwayo ibonise ukukhiqiza kabusha okuhle kakhulu, ukuzinza, nokukhetha okuvelele, futhi i-ATP ingahlukaniswa ngempumelelo kuma-analogue ayo.Okubaluleke nakakhulu, leli su le-aptasensor le-ECL elisebenzayo elisuselwe ku-GO esebenza kokubili njengenkomba ye-electrochemical kanye nesignali ye-ECL livamile futhi linganwetshwa kalula kweminye imicimbi ebophezelayo yezinto eziphilayo.