Imikhiqizo

I-2,3,4,6-Tetrakis-O-trimethylsilyl-D-gluconolactone, ngokuvamile eyaziwa ngokuthi i-TMS-D-glucose, iyinhlanganisela eguquguqukayo ethola izinhlelo zokusebenza emikhakheni ehlukahlukene yesayensi, kuhlanganise ne-organic synthesis, i-carbohydrate chemistry, kanye nekhemikhali yokuhlaziya. I-TMS-D-glucose ibaluleke kakhulu ku-organic synthesis njengoba isebenza njengeqembu elivikelayo lamaqembu asebenzayo e-hydroxyl (OH) kuma-carbohydrate.Ngokwethula amaqembu e-trimethylsilyl (TMS) emaqenjini e-hydroxyl eglucose, inhlanganisela iba izinzile futhi ingasebenzi kancane, okuvumela ukuguqulwa okukhethiwe kwamaqembu athile e-hydroxyl ngenkathi ishiya abanye bengathinteki ngesikhathi sokuguqulwa kwamakhemikhali okwalandela.Leli su lokuvikela-lokuvikela lisetshenziswa kakhulu kukhemikhali ye-carbohydrate ukuze kuzuzwe ukukhethwa kabusha okufiselekayo kanye ne-stereochemistry ekuhlanganiseni ama-carbohydrate ayinkimbinkimbi, ama-glycoconjugates, nemikhiqizo yemvelo.Ekhemistry yokuhlaziya, i-TMS-D-glucose isetshenziswa njenge-reagent ephumayo ukuze kutholwe kanye nokulinganisa. ama-carbohydrate.Ngokuguqula ama-carbohydrate abe yimikhiqizo yawo ye-trimethylsilyl, ukuntengantenga kwawo kanye nokuzinza kokushisa kuyathuthuka, okuwenza afanelekele ukuhlaziywa nge-gas chromatography (GC) kanye ne-mass spectrometry (MS).Le nqubo yokukhipha ithuthukisa ukuzwela kokutholwa, ithuthukisa ukusebenza kahle kokuhlukana, futhi inike amandla ukukhonjwa kwama-carbohydrate ahlukahlukene kuzingxube eziyinkimbinkimbi, njengamasampula ebhayoloji noma imikhiqizo yokudla.I-TMS-D-glucose iphinde ithole izinhlelo zokusebenza ekuhlanganiseni kwezinto ezisizayo ezikhethekile nama-chemical probes.Ukusebenza kabusha kwayo okuyingqayizivele nokuzinza kwayo kuyenza ibe yinto yokuqala ebalulekile yokulungiselela ezinye izinhlanganisela ezitholakala kuma-carbohydrate.Abacwaningi bangashintsha i-trimethylsilyl moiety noma bafake esikhundleni se-glucose moiety ukuze benze izinhlanganisela ezinezakhiwo ezithile, njengama-fluorescent probes, ama-enzyme inhibitors, noma amakhandidethi ezidakamizwa.Lezi ziphuma zingasetshenziswa ezifundweni ezihlukahlukene zebhayoloji nezamakhemikhali, okuhlanganisa ukuthwebula izithombe, ukuthuthukiswa kwezidakamizwa, noma ukuqonda ukusebenzisana kwe-carbohydrate-protein.Nokho, kubalulekile ukucabangela ukuthi i-TMS-D-glucose, njenganoma iyiphi enye inhlanganisela yamakhemikhali, idinga ukuphathwa ngendlela efanele nokuphepha. izinyathelo zokuzivikela.Abacwaningi kufanele baqinisekise ukungena komoya owanele futhi basebenzise izinto zokuzivikela ezifanele lapho besebenza nale nhlanganisela ukuze bagweme izingozi ezingaba khona empilweni.Ukwengeza, njenganoma iyiphi i-reagent yamakhemikhali, ubumsulwa, kanye nekhwalithi ye-TMS-D-glucose ibalulekile ukuze uthole imiphumela enokwethenjelwa futhi ekwazi ukukhiqiza kabusha.Ikhono layo lokuvikela ngokukhethekile amaqembu e-hydroxyl kuma-carbohydrate, ukusebenza kwawo ekuhlaziyeni ama-carbohydrate, kanye nokusetshenziswa kwawo ekuhlanganiseni ama-reagents akhethekile kuyenza ibe ithuluzi elibalulekile emikhakheni eyahlukene yesayensi.Ngokusebenzisa i-TMS-D-glucose, abacwaningi bangaqhubekisela phambili izifundo zabo kukhemikhali ye-carbohydrate, i-glycoscience, nezinkambu ezihlobene, okunikela ekuthuthukisweni kwamakhompiyutha amasha, ukuxilonga, nama-ejenti okwelapha.