Imikhiqizo

I-protein phosphorylation ilawula imisebenzi ehlukahlukene yamaselula futhi idlala indima ebalulekile ekuthuthukisweni kokuqala kwezitshalo.Ukugcwalisa nokwandisa ophenyweni lwangaphambilini lwe-protein phosphorylation ezitshalweni ze-Arabidopsis sisebenzise enye indlela ehlanganisa ukukhishwa kwamaprotheni ngaphansi kwezimo ezingezona ze-denaturing ne-immobilized metal-ion affinity chromatography (IMAC) yokuthuthukisa ama-phosphoprotein aqinile ku-Rubisco-depleted extracts, okulandelwa ukuhlonza. kusetshenziswa ijeli ye-electrophoresis enezinhlangothi ezimbili (2-DE) kanye ne-liquid chromatography-tandem mass spectrometry (LC-MS/MS).Ukugaya i-trypsin kujeli kanye nokuhlaziywa kwamabala ejeli akhethiwe kukhonjwe ama-peptides nezinsalela ze-phosphorylated eziyi-144, okungama-phosphopeptides angu-18 kuphela nama-phosphosites angu-8 atholakala kusizindalwazi sesizindalwazi se-PhosPhat 4.0 kanye ne-P3DB Arabidopsis thaliana phosphorylation.Ngaphezu kwengxenye ye-82 ehlonziwe i-phosphoprotein yayihileleke ku-carbohydrate metabolism, i-photosynthesis / ukuphefumula noma i-oxidative stress response mechani sms.Ukunothiswa kwama-phosphoprotein aqinile ngaphambi kwe-2-DE kanye ne-LC-MS/MS kubonakala kuthuthukisa ukutholwa kwezinsalela ze-phosphorylated threonine kanye ne-tyrosine uma kuqhathaniswa nezindlela ezisebenzisa ukunothisa kweleveli ye-peptide, okuphakamisa ukuthi lezi zindlela ezimbili ziyahambisana ngandlela thize mayelana nokumbozwa kwesayithi le-phosphorylation.Ukuqhathanisa imiphumela yezithombo ezisencane naleyo etholwe ngaphambilini kumaqabunga e-Arabidopsis avuthiwe kuhlonzwe amaprotheni amahlanu ane-phosphorylated ngokuhlukile kulezi zicubu, okubonisa amandla ale nqubo yokuphenya amandla e-protein phosphorylation ngesikhathi sokukhula kwezitshalo.