N,N'-Methylenebisacrylamide Cas: 110-26-9 98% Impushana emhlophe
| Inombolo Yekhathalogi | XD90231 |
| Igama Lomkhiqizo | N,N'-Methylenebisacrylamide |
| I-CAS | 110-26-9 |
| I-Molecular Formula | I-C7H10N2O2 |
| Isisindo samangqamuzana | 154.1665 |
| Imininingwane Yesitoreji | 2 kuya ku-8 °C |
| Ikhodi Yentela Evumelanisiwe | 29241900 |
Ukucaciswa Komkhiqizo
| Isivivinyo | >98% |
| Amanzi angancibiliki | <0.5% |
| Ukubukeka | Impushana emhlophe |
| Ama-sulfate e-inorganic | <0.5% |
| Okokusetshenziswa kocwaningo kuphela, hhayi okomuntu | ukusetshenziswa kocwaningo kuphela, hhayi ukusetshenziswa komuntu |
Indima ebalulekile ye-tumor-associated fibroblasts (TAF) ekuqhubekeleni phambili komdlavuza manje isicacile kumdlavuza wamaphaphu ongewona omncane (NSCLC).Kodwa-ke, izindlela zokwelapha ezimelene nama-TAF zikhawulelwe ngenxa yokuntula ukuqonda ezindleleni ezithize ze-subtype eziyisisekelo zokunqwabelana kwazo.Lapha, ukuqina komshini (okungukuthi, ukuqina kwe-matrix) kanye nezimpawu ze-mitogenic ezincibilikayo eziqhuba ukunqwabelana kwama-TAF kusuka ezinhlotsheni ezincane ze-NSCLC: i-adenocarcinoma (ADC) kanye ne-squamous cell carcinoma (SCC) zahlakazwa.Ama-fibroblasts akhuliswe ku-substrata eklanyelwe ukukhombisa ukuqina okujwayelekile- noma okufana ne-tumor ekugxilweni okuhlukene kwe-serum, futhi izinqubo zokulawula ezibalulekile zacaciswa.Ekulawuleni ama-fibroblasts asuka kuzicubu ezingenabungozi, ukuqina kwe-matrix kukodwa kukhuphule ukunqwabelana kwe-fibroblast, futhi lo mphumela wokusebenza wawunamandla noma uqhathaniseka nalowo wezici zokukhula ezincibilikayo kufika ku-0.5% serum.Izimpawu ezishukumisayo zokuqina kwe-matrix zaziqhutshwa yi-β1 integrin mechano-sensing nge-FAK (pY397), futhi zazihlotshaniswa nokukhuphuka okuqhutshwa ngemva kokubhala kombhalo ku-β1 integrin expression.Isekethe yokugcina ye-mechano-regulatory nayo yabonwa kuma-TAF kodwa ngendlela ethize ye-subtype, ngoba ama-SCC-TAF abonise i-FAK ephezulu (pY397), i-β1 expression, kanye ne-ERK1/2 (pT202/Y204) kune-ADC-TAFs.Ngaphezu kwalokho, ukuqina kwe-matrix kubangele ukunqwabelana kwe-TAF enkulu kuma-SCC-TAF (>50%) uma kuqhathaniswa nama-ADC-TAF (10% -20%).Ngokuphambene, ama-SCC-TAF ayengazweli kakhulu ku-serum, kuyilapho ama-ADC-TAF asabela ekugxilweni okuphezulu kwe-serum kuphela.Lokhu okutholakele kunikeza ubufakazi bokuqala bokulawulwa kwe-subtype-specific of NSCLC-TAF accumulation.Ngaphezu kwalokho, le datha isekela ukuthi imithi yokwelapha ehloselwe ukubuyisela ukunwebeka okujwayelekile kwamaphaphu kanye/noma ukulawulwa kwe-mechano encike ku-β1 kungase kusebenze ngokumelene nama-SCC-TAFs, kuyilapho ukuvimbela ukusayinda kwesici sokukhula kwe-stromal kungase kusebenze ngokumelene nama-ADC-TAFs.Lolu cwaningo lwembula izindlela ezihlukile eziyisisekelo ukunqwabelana okungavamile kwama-fibroblast asekela isimila ezinhlotsheni ezincane ezimbili ezinkulu zomdlavuza wamaphaphu, okuzosiza ukuthuthukiswa kwezindlela zokwelapha eziqondene nomuntu ngokumelene nalawa maseli.
