I-PMSF Cas: 329-98-6 98.0% i-crystalline powder emhlophe i-Phenylmethanesulfonyl fluoride (PMSF)
| Inombolo Yekhathalogi | XD90250 |
| Igama Lomkhiqizo | I-Phenylmethanesulfonyl fluoride (PMSF) |
| I-CAS | 329-98-6 |
| I-Molecular Formula | I-C7H7FO2S |
| Isisindo samangqamuzana | 174.1927 |
| Imininingwane Yesitoreji | I-ambient |
| Ikhodi Yentela Evumelanisiwe | 29049900 |
Ukucaciswa Komkhiqizo
| Isivivinyo | ≥98.0% HPLC |
| Ukubukeka | i-crystalline powder emhlophe |
I-PMSF iyi-serine/cysteine protease inhibitor engaphenduki evame ukusetshenziswa ekulungiseleleni ama-cell lysates.
Izifundo ze-in vitro: I-PMSF (2 mM) ivimbele ukunqwabelana kwe-inositol phosphate evuselelwe i-carbachol ngo-15% -19% kuphela lapho kukhona i-Li+.Ukuvinjelwa kokushintshwa kwe-phosphoinositide yi-PMSF kungenxa yesinyathelo esisodwa noma ngaphezulu kulandela ukuwohloka kwe-phosphoinositides [1].I-PMSF ivimbela i-acylation yezinsalela ze-inositol ze-GPI ezimaphakathi egazini le-T. brucei.I-PMSF ivimbela ukwakheka kwe-glycolipid C kodwa hhayi ukuguqulwa kabusha kwe-fatty acid in vitro.I-PMSF ivimbela ukungezwa kwe-GPI acylation kanye ne-ethanolamine phosphatase kuma-procyclic trypanosomes, kodwa hhayi kumaseli we-Hela [2].
Izifundo ze-in vivo: I-PMSF (0.1 mL/10 g b.wt, ip) ikhiqize i-antinociception, njengoba kuboniswa ukwenyuka kokusabela komthamo ku-%MPE ekuhloleni ukubambezeleka komsila, kodwa yehlulekile ukukhiqiza ukuvinjelwa kwemoto okuphendula umthamo okucacile.Amagundane athole umjovo we-intraperitoneal we-PMSF abonise imiphumela ye-cannabinoid ehlanganisa i-antinociception, i-hypothermia kanye nokungahambi kahle ngamavelu e-ED50 we-86, 224 kanye ne-206 mg / kg, ngokulandelana.Ukwelashwa kwangaphambili kwe-PMSF (30 mg/kg) kuthuthukisa imiphumela ye-anandamide ezimpendulweni ze-tail-flick (i-antinociception), umsebenzi we-locomotor nokuhamba nge-5-fold, 10-fold kanye ne-8-fold, ngokulandelana [3].
Ukuhlolwa kwezilwane: Amagundane e-ICR abesilisa anesisindo esingu-18 kuya ku-25 g asetshenziswe ekuhlolweni.I-PMSF yahlakazwa kuwoyela wesesame futhi yafakwa ngaphakathi kwe-intraperitoneally ngevolumu engu-0.1 mL/10 g b.wt.Njalo sebenzisa i-PMSF imizuzu eyi-10 ngaphambi komjovo we-anandamide noma wemoto.Amagundane ajwayela igumbi lokuhlola ubusuku bonke ngaphandle kokuphazamiseka kokudla noma amanzi.Ukulandela i-anandamide ye-intravenous noma ukuphathwa kwemoto, isilwane ngasinye sihlolwe kanje: imizuzu ye-5 yempendulo ye-tail-flick latency (antinociceptive) kanye ne-5 kumaminithi we-15 wokusebenza okuzenzakalelayo (motor);noma imizuzu emi-5 yezinga lokushisa elingumongo (rectal) kanye ne-Immobilization (catalepsy) iringi imizuzu emi-5 ukuya kweyi-10.
