Imikhiqizo

I-Chemifluorescence: I-molecular luminol i-molecule ye-fluorescent yamakhemikhali engaguqulwa ibe yi-aminophthalic acid yesimo esijabulisayo phambi kwama-molecule e-hydrogen peroxide, ekhipha i-fluorescence eqinile.I-hydrogen peroxide iwumkhiqizo wokusabela okuningi kwe-biooxidative, ngakho-ke kulula ukuhlanganisa lokhu kusabela kwe-biooxidative ne-photodetection ngokwethula i-luminol.Isibonelo, i-glucose oxidase/catalase probe ingathola ukugcwala kwe-hydrogen peroxide noma i-glucose kusampula, futhi isikhathi sokuphendula singu-0.5s kuphela (indlela eguqukayo).Ukuhlanganisa izinto ze-chemiluminescent nokusabela kwe-immunoassay, nokuveza ukugxila kwezakhi zokuzivikela ezitholiwe ngokusabela kokukhanya, kubizwa ngokuthi ubuchwepheshe be-chemiluminescent immunoassay.Ngo-1976, u-Shmeder wenza isiphakamiso se-chemiluminescence immunoassay, esebenzisa i-luminol-H202 kanye nokuphuma kwayo kwe-ABEI njengohlelo lokubonisa ukusabela kokubona.Njengamanje, i-luminol kanye nokuphuma kwayo kuyizimpawu ezisetshenziswa kakhulu ezinhlelweni ze-chemiluminescence immunoassay.Ngaphansi kwezimo ze-alkaline, i-catalyzed by microperoxidase, inani elikhulu lama-photon lingakhululwa.I-Luminol ingasetshenziselwa i-WesternBlot yamasosha omzimba anelebuli ye-HRP kanye nokutholwa kwe-nucleic acid hybridization yama-probe anelebuli ye-HRP.Iphinde isetshenziswe ekutholweni kwe-bloodstain yobugebengu besimanje.I-compound engasetshenziswa njenge-chemiluminescent marker kufanele ihlangabezane nezimo ezilandelayo: isivuno se-quantum se-luminescence siphezulu;izici zayo ze-physicochemical zihambisana nesistimu efundwayo;ukusabela kwayo kwe-luminescent kuwumphumela wokusabela kwe-oxidation yento ekhanyayo;ngaphakathi kwebanga lokuhlushwa elisetshenziswa Ngaphakathi ezingenabo ubuthi ezintweni eziphilayo.Izinhlobo eziningana ze-chemiluminescence reagents zivame ukusetshenziswa: i-acridine ester, i-chemiluminescence tracer esetshenziswa kabanzi, iyinhlanganisela ye-tricyclic ephilayo, kulula ukuyi-oxidize, futhi ukusabela kwe-oxidation akudingi i-catalyst, futhi ikhulula ama-photons ku-430nm;I-Luminol ne-isoluminol kanye nokuphuma kwayo kungamakhemikhali avuthiwe kakhulu.Kusukela ngo-1964, Ngenkathi kubikwa nge-chemiluminescence, i-luminol, i-isoluminol kanye nokuphuma kwayo kwasetshenziselwa i-chemiluminescence.Ukuhlolwa kwe-immunoassay kuzuze imiphumela engcono.Ukutholwa kwe-Luminescence: ubude beza beza be-fluorescence obulungile ngu-400nm (ukutholwa kwe-chemiluminescence ku-60mmK2S2O8, 100mmK2CO3, isixazululo se-pH11.5)

I-Chemiluminescence: Njengendlela yokuhlola ebucayi, i-chemiluminescence isetshenziswa kabanzi ukuthola ama-radicals mahhala kanye nama-metabolites okusabela akhiqizwa kuma-enzyme, amaseli kanye nezinto eziphilayo.Ukukhanya okukhishwa imikhiqizo yabo ye-oxidation kanye nama-metabolites kungasetshenziswa nama-photometer ahlukahlukene.ukutholwa.I-Chemiluminescence isetshenziswa kabanzi ekuhlolweni nasekucwaningweni kwama-antioxidants, njenge-polyphenols, i-polysaccharides, i-flavonoids, nama-anthraquinones, ngenxa yokuzwela kwayo, ukusheshisa, ukusebenza okulula kanye nenani eliphansi.Amasistimu e-chemiluminescence asetshenziswa kakhulu ekunqumeni ama-anion e-superoxide ahlanganisa i-xanthine oxidase-luminol, i-pyrogallol-luminol ne-dimethyl sulfoxide-luminol.Olokuqala luwuhlelo lwe-enzymatic, kanti olubili lwakamuva luwuhlelo olungenalo i-enzymatic.Izinhlelo ze-chemiluminescence zokunqunywa kwe-hydroxyl radicals ikakhulukazi zihlanganisa i-copper sulfate-yeast (noma amaseli omnkantsha) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, copper sulfate-o-phenanthroline-ascorbic acid-hydrogen I-peroxide 5 chemiluminescence systems, i-ferrous sulfate-luminol-hydrogen peroxide kanye ne-ferrous sulfate-luminol, konke kuhilela ukusabela kwakudala kwe-Fenton ukuze kukhiqizwe ama-radicals e-hydroxyl, bese ehlasela i-agent ekhanyayo ukuze ikhiqize i-chemiluminescence, engasetshenziswa ukukala ukukhishwa kwe-extracts. umsebenzi wama-oxygen radicals asebenzayo.Indlela yokuhlola: sebenzisa uhlelo lwe-pyrogallol-luminol chemiluminescence: i-luminol yenziwe ibe yisisombululo sokuhlushwa esingu-0.05mol/L esinesixazululo esingu-0.05mol/L NaOH, sigcinwe endaweni emnyama, namanzi acwecwe kabili ngaphambi kokusetshenziswa Dilute to 1mmol/L solution, sebenzisa 1mmol/ L HCl ukwenza isixazululo esingu-0.01mol/L se-pyrogallol, sigcine esiqandisini ngo-4°C, bese usihlanza ngamanzi acwecwe kabili abe yisixazululo esingu-16 izikhathi ezingu-6.25×10-4mol/L ngaphambi kokusetshenziswa.I-0.05mol/L pH10.2Na2CO3-NaHCO3 buffer (equkethe u-0.1mmol/LEDTA) yalungiswa ngaphambi kokusetshenziswa, futhi yaxutshwa ne-1mmol/L luminol ngo-2:1 (ingxenye yevolumu) ukuze kwakheke i-luminol ne-carbonate buffer ngaphambi kwengxube yokuhlola.Phakathi nesilinganiso, jova amasampuli angu-10.0 μL wokugxilisa okuhlukile (0, 0.08, 0.4, 2 kanye no-10 mg/mL) kuseli elikhanyayo (ngesampula yebhafa njengokulawula), bese ujova 6.25×10-4mol/L pyrogallol. 0.05 Ekugcineni, u-0.94 mL wengxube ye-luminol ne-carbonic acid buffer yengezwe ukuze kuqalwe ukusabela (30°C), amandla e-luminescence abalwe ngezikhawu zamasekhondi angu-2, futhi inani eliphelele lokuqina kwe-luminescence elihlanganisiwe ku-300 s likalwa.Ukuqina kwe-luminescence yangemuva bekuyi-luminescence lapho i-pyrogallol ingangeziwe.inani.Ngaphezu kwalokho, kukhona i-chemiluminescence, i-fluorescence spectroscopy kanye ne-NBS-dichlorofluorescein chemiluminescence uhlelo lokunqunywa kwe-fluorescence chemiluminescence, njll., inzuzo yabo enkulu ukuzwela okukhulu.

Izakhiwo Zamakhemikhali: Impushana yekristalu ephuzi.Incibilika kalula ku-lye, incibilika ku-asidi encibilikayo, cishe ayincibiliki emanzini, futhi ayincibiliki otshwaleni.Izixazululo ezingathathi hlangothi noma ezine-acidic kancane zibonisa i-fluorescence egqamile eluhlaza okwesibhakabhaka lapho zivezwe ekukhanyeni kwe-ultraviolet.Iphoyinti lokuncibilika 329-332 ℃

Ukusetshenziswa: Njenge-reagent yokuhlaziya amakhemikhali kanye nenkomba.Sebenzisa i-Chemiluminescence reagent yokuthola ukuhlaziya (njengokunqunywa kwama-cations ensimbi noma igazi)

Ukusetshenziswa: Ukuhlaziywa kwe-chemiluminescence, okufana: i-cations yensimbi, igazi nama-glucocorticoids

Ukusetshenziswa: Ukuhlolwa kwe-Luminescence: I-Emmax440nm (i-chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; ngemva kokwengeza i-H2O2) i-chemiluminescence reagent kanye nenkomba, evame ukusetshenziswa ekuhlaziyweni kwe-chemiluminescence, njengama-cations ensimbi, ukungatheleleki kwegazi, njll.