4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99%
| Inombolo Yekhathalogi | XD900018 |
| Igama Lomkhiqizo | 4-Nitrophenyl-alpha-D-galactopyranoside |
| I-CAS | 7493-95-0 |
| I-Molecular Formula | I-C12H15NO8 |
| Isisindo samangqamuzana | 301.25 |
| Imininingwane Yesitoreji | -15 kuya ku-20 ° C |
| Ikhodi Yentela Evumelanisiwe | 29400000 |
Ukucaciswa Komkhiqizo
| Ukubukeka | Okumhlophe kuya ku-Orenji kuya kumpushana Okuhlaza kuya kukristalu |
| Isivivinyo | 99% |
I-4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) i-substrate yokwenziwa ye-4-nitrophenyl (pNP) glycopyranoside ukuze kutholwe umsebenzi we-α-galactosidase.Inani le-pNP ekhishiwe lenyuke kakhulu lapho i-4-Nitrophenyl α-D-galactopyranoside isetshenziswa njenge-substrate.
Ukusebenzisa i-4-nitorophenol (pNP) -i-glycopyranosidesaspseudosubstrates, inani elikhululiwepNPkusukela ku-4-Nitrophenylα-D-galactopyranoside liphakeme kakhulu kunokwenye i-NP-glycopyranosidesatpH4.0lapho kungekho-umsebenzi wegazi-ushicile4-ushicile. I-nyl (pNP) i-glycopyranosidesasa-substrates yokwenziwa ikhombisa ukuthi i-galactoseresidues ekhululiwekusuka ku-4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, okungukuthi i-Hlevelwhereyieldthresholdiyancipha kakhulu.
I-Substrate ye-α-D-galactosidase (α-D-Galactosidase).Ibophezela ku-E. coli lactose protein carrier.Okumaphakathi kwe-α-D-galactose.
Indlela esheshayo yokuhlola umsebenzi we-lytic wama-antimicrobial agents ngokumelene nemvubelo nesikhunta iye yasungulwa.Ukuhlolwa kusekelwe ekukhishweni kwe-enzyme ye-intracellular, i-maltase (i-alpha-glucosidase).Umsebenzi okhululiwe we-maltase ukalwe ngokombala ngokukhiqizwa kwe-p-nitrophenol kusuka ku-p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Umsebenzi we-lytic wezinhlanganisela ezihlukene zama-antimicrobial ukalwe ngokumelene namaseli emvubelo noma izinhlamvu ezikhulayo zesikhunta esine-filamentous.Umsebenzi we-Lytic anti-yeast ungatholwa phakathi kwemizuzu engama-20 ekufukameleni ku-30 degrees C uma kuqhathaniswa ne-Saccharomyces cerevisiae, i-Candida albicans, ne-Cryptococcus neoformans.Umsebenzi we-Lytic anti-fungal uvele ngemva kwamahora angu-2 wokufukamela ku-30 degrees C ngokumelene nezinhlamvu ezikhulayo ze-Aspergillus niger ne-Botrytis cinerea.Wonke amaseli weyeast noma isikhunta awazange akhiqize i-PNPG eyanele nge-hydrolyze phakathi kwamahora angu-3 ku-30 degrees C ukuze aveze ushintsho olubonakalayo lombala.Umsebenzi we-lytic wama-enzyme (isb., i-Lyticase), ama-antibiotics (isb., i-Amphotericin B), kanye nohlobo lwamagciwane akhiqiza ama-antibiotic atholwe kusetshenziswa ukulinganisa.I-anti-yeast assay iguqulelwe kufomethi ye-microtiter engu-96-well.Kokubili ukulinganisa kunikeze ukutholwa okusheshayo, okuzwelayo, nokuphindaphindeka komsebenzi we-lytic anti-yeast nomsebenzi wokulwa nesikhunta.
